Effects of secondary compounds from cactus and acacias trees on rumen microbial profile changes performed by Real- Time PCR
Abstract: Plant rich secondary compounds had antimicrobial effects by acting against different rumen microbial populations. The current study investigated the influence of spineless cactus (Opuntia ?cus indica f. inermis), Acacia nilotica and A. saligna on rumen microbial fermentation, using in vitro gas production technique, and microbial population profile changes, using a molecular-based technique (Real-Time PCR). The acacias and Opuntia reduced significantly total gas production (p?0.01), rumen CH4 production (p?0.01) and ammonia concentration (p?0.001). At 24h of incubation, Fungi population was 0.30- and 0.03 -fold reduced with A.nilotica and Opuntia as compared to 0h, but 2-and 1.24- fold higher with A.cyanophylla .Increases in the abundance of F.succinogenes were observed in all substrates; however, the tanniferous plants and Opuntia reduced the relative abundance of R.flavefaciens. Methanogenic population was increased with all substrates, except for Opuntia (0. 90- fold lower than the control). There was a significant reduction (p<0.05) in rumen protozoa count with A.cyanophylla, Opuntia and A.nilotica (3.68; 5.59 and 5.34 times, respectively). Results suggested that tannin sources from A.nilotica and A.cyanophylla had an indirect effect on methanogenesis. This study showed an antimicrobial activity of oxalates content of O. ficus indica.
[Chentli Amira, Gilmann Louisa, Bouazza Lyas, Medjkal Samir, Limami Anis Mohamed, Le Paven Marie-Christine Morère, Bousseboua Hacène (2014); Effects of secondary compounds from cactus and acacias trees on rumen microbial profile changes performed by Real- Time PCR Int. J. of Adv. Res. 2 (2). 0] (ISSN 2320-5407). www.journalijar.com
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