17Sep 2016

MOLECULAR DETECTION OF ANTIBIOTIC RESISTANCE GENE (MECA) IN STAPHYLOCOCCUS EPIDERMIDIS LOCAL ISOLATES.

  • Ministry of Higher Education and Scientific Research- University of Baghdad - Genetic Engineering and Biotechnology Institute for Postgraduate Studies. Iraq-Baghdad.
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The mecAgene is a gene found in bacterial cells. The mecAgene allows a bacterium to be resistant to antibiotics such as methicillin, penicillin and other antibiotics. This study is based on the molecular analysis of the antibiotic resistance gene (mecA). The polymorphism of the mecAgene existing in different S. epidermidishave been analyzed in this study. Seventy five isolates (57) of S.epidermidiswere obtained from eighty four (84) which were collected three hospitals in Baghdad include Al-Yarmuk General Teaching Hospital , Al-Karama teaching hospital, Ibn-ALbalady teaching hospital. These isolates were collected from clinical sources the samples included, wound swab, burn swab, ear swab and urine. .DNA from each sample was isolated and the mecAgene was amplified with appropriate primers. The PCR results of 57 mecA gene positive S.epidermidisgave the band size of amplicons about 500pb.The presence of mecAgene seems to be enhanced in biofilm producers and enable S.epidermidis strains to show increasing resistance to different groups of antibiotics.


[Rusul Muzher Hussein and Marrib Nazih Rasheed. (2016); MOLECULAR DETECTION OF ANTIBIOTIC RESISTANCE GENE (MECA) IN STAPHYLOCOCCUS EPIDERMIDIS LOCAL ISOLATES. Int. J. of Adv. Res. 4 (Sep). 506-510] (ISSN 2320-5407). www.journalijar.com


Rusul Muzher Hussein


DOI:


Article DOI: 10.21474/IJAR01/1510      
DOI URL: http://dx.doi.org/10.21474/IJAR01/1510