PLASMA LEVEL OF TOTAL ANTIOXIDANT AND FREQUENCY OF HBSC, HBSS,HBAA, HBAS, HBAC AND G6PD DEFICIENCY IN ICTERIC CHILDREN PRESENTING WITH ANAEMIA.

Background:- Jaundice is a common clinical condition among anaemic children. Aim and Objective:- This work was designed to evaluate the Plasma Level of Total Antioxidants and Frequency of HbSC, HbSS, HbAA, HbAS, HbAC and G6PD Deficiency in Icteric Children Presenting with Anaemia. Materials and Methods:- One hundred icteric children aged 3-12 years presenting with anaemia were recruited for this work from the General Hospital, Oka-Akoko-Ondo state.Alkaline cellulose acetate membrane haemoglobin electrophoresis was carried out on the subjects to determine the haemoglobin types while immunochromatographic technique was adopted was qualitative determination of G6PD. ELIZA techchnique was used to determine HBsAg and anti-HCV in the subjects.Cyaemethhaemoglobin method was used to determine haemoglobin concentration and capillary tube method was used to evaluate PCV.Total Antioxidantswas determined in the subjects biochemically by spectrophotometry. Results:- The result obtained in the icteric children presenting with anaemia showed a significant reduction in the haemoglobin concentration in the icteric children with G6PD deficiency than the haemoglobin concentration and PCV value obtained in icteric children with HbAS, HbAC and HbAA. The PCV value obtained in icteric children with G6PD deficiency was significantly higher than in HbSC. The frequencies of G6PD deficiency and electrophoretic types in the icteric children were found to be: 40(40%) G6PD deficiency, 28(28%) HbSC,30(30%)HbSS,2(2%) HbAS, 2(2%) HbAC, and 2(2%) HbAA respectively. While thepattern of gender distribution obtained from the study showed: Male:30(30%) G6PD deficiency, 12(12%)HbSC,16(16%) HbSS, 0(0%) HbAS, 1(1%) HbAC, 0(0) HbAA and in female: 10(10%)G6PD deficiency, 16(16%)HbSC, 14(14%) HbSS, 2(2%) HbAS, 1(1%)HbAC and 2(2%) HbAA. G6PD deficiency and HbSS were more pravalent in icteric male children while HbAS and HbAA were more frequent in their female counterpart.There was a significantly lower plasma value of Total antioxidant in patients HbSS, HbSC and those with G6PD deficiency than the value obtained in subjects with HbAA, HbAS and HbAC with p<0.05. There was a significantly lower plasma value of Total antioxidant in HbSS, HbSC than those with G6PD deficiency with p<0.05. Conclusion: G6PD deficiency, HbSS and HbSC with significant reduction in total antioxidant were more prevalent among the icteric children.