23Nov 2016

MOLECULAR DETECTION OF GYRA AND PARC GENES IN MULTIDRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM NOSOCOMIAL INFECTIONS IN CHILDREN.

  • M Sc student, Children Welfare Teaching Hospital, Medical City, Baghdad, Iraq.
  • PhD Medical Microbiology, Middle Technical University, College of Health & Medical Technology, Dept. of Medical Laboratory Technology, Baghdad, Iraq.
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This study was conducted in Children Welfare Teaching Hospital, Baghdad, Iraq in order to detect gyrA and parC genes in multidrug resistant Acinetobacter baumannii isolated from nosocomial infections in children. A total of 50 child patients were selected according to study criteria. Different specimens obtained from child patients and hospital environment were tested by culture and antimicrobial sensitivity according to standard bacteriological and molecular techniques. Blood stream infections comprised the highest number and percentages within suspected nosocomial infections, 21 (42%) of paediatric patients were detected with early onset sepsis (EOS), and 19 (38%) were with late onset sepsis (LOS). Forty A. baumannii isolates were identified at species level by using VITEK® 2 Compact and 16s rRNA. Oligonucleotide primers sequences were used for PCR amplification of 16s rRNA , gyrA and parC of Acinetobacter baumannii. All of A. baumannii isolates were multidrug resistant (MDR) pathogens. Resistance gyrA and parC genes were detected in 58% and 100% of study isolates, respectively. Nosocomial sepsis in neonates were still at high-frequency in child patients. The vast majority of A. baumannii isolates were multidrug resistant and harboring gyrA and parC resistance genes. Considerable efforts are needed for choosing antimicrobial drugs and combination therapy. Further studies are required for genotyping by sequencing of problematic resistant A. baumannii isolates in our hospitals.


[Ban Imad Abbood and Falah Salim Manhal. (2016); MOLECULAR DETECTION OF GYRA AND PARC GENES IN MULTIDRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM NOSOCOMIAL INFECTIONS IN CHILDREN. Int. J. of Adv. Res. 4 (Nov). 57-65] (ISSN 2320-5407). www.journalijar.com


Dr. Falah Salim Manhal


DOI:


Article DOI: 10.21474/IJAR01/2055      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2055