SCREENING AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF THYMUS VULGARIS USING IN VIVO AND IN VITRO METHODS

There is a great effort to discover plant-derived antibacterial against multi-drug resistant bacteria. The objective of the study to the possibility of organic production of Thymus vulgaris herb under sandy soil and investigate the in vitro and in vivo antibacterial activities of thyme essential oil that was analyzed and its constituents were identified by GC/MS. The major components were thymol (45.15%) and p-cymene (25.31%), borneol (5.06%), myrecen (3.42%) and ?-pinene (3.21%). A Staphylococcus aureus-infected mice model was subjected to meet the study objective. The physiological, biochemical and histopathological markers of possible effects of this essential oil were studied using various standard methods. The extract had a significant effect on the number of viable Staphylococcus aureus recovered from spleen where 4MIC group was the best group in inhibiting the bacteria followed by 2MIC and 1MIC. There were no side effects on vital organs observed even in using 4MIC dose. The obtained results indicate that the essential oil of thyme has the potential to provide an effective treatment for S. aureus infection. It is necessary to further investigate the interaction between the pathogenic bacteria and thyme essential oil and its impact on the development of resistance.