30Nov 2016

ENHANCING MEDIA COMPOSITIONS FOR NATTOKINASE PRODUCTION: AN APPROACH OF RESPONSE SURFACE METHODOLOGY

  • Ahfad University for Women.
  • Faculty of Agriculture, University of Khartoum.
  • Central Lab Ministry of Higher Education and Scientific Research.
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This study aimed to isolate and characterize a potent Bacillus strain to produce a fibrinolytic enzyme, nattokinase, with highest activity under optimal media compositions. The study was carried out on 30 samples collected from different regions in Sudan. Primary screening of the microorganism showed that sixteen out of thirty samples (53%) showed a clear large inhibition zone in casein hydrolysis and blood heamolysis methods. Ten out of sixteen (63%) of the selected sample considered as Bacillus subtilis according to morphological, microscopic and biochemical analysis. Selective medium was prepared for the extraction and production of nattokinase from these new isolates. Response Surface Methodology (RSM) was employed to optimize a fermentation medium for the production of nattokinase by Bacillus subtilis at pH7.2, temperature of 37ºC and agitation rate of 140 rpm. The four variables involve in this study were glucose, maltose, peptone and yeast extract and consistent MgSo4 and K2HPO4. The result showed that the peptone had no a significant effect on nattokinase production the optimized medium containing (%) glucose 1.45, maltose 5.5, yeast extract 2.75 and no level of peptone incorporated to the medium. The predicted response of nattokinase activity will be of 0.827917 Fibrinolytic Unit (FU).


[Wegdan Abd Al Rahman Hamadan, Aisha Mudawi Alawad, Elrashied E. Elkhidir and Hanan Moawia Ibrahim. (2016); ENHANCING MEDIA COMPOSITIONS FOR NATTOKINASE PRODUCTION: AN APPROACH OF RESPONSE SURFACE METHODOLOGY Int. J. of Adv. Res. 4 (Nov). 1536-1546] (ISSN 2320-5407). www.journalijar.com


Wegdan Abd Al Rahman


DOI:


Article DOI: 10.21474/IJAR01/2244      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2244