AN ATTEMPT TO REDUCE THE TOXICITY OF BISPHENOL A BY USING PROBIOTIC DRUG THERAPY- A STUDY OF 2 D PAGE.

Bisphenol A (BPA) is a chemical extensively used in the manufacture of polycarbonate plastics and epoxy-phenolic resins. The human population is widely exposed to BPA, primarily by way of the diet by migration from food and beverage containers. In the present study, we were isolated the hypothalamus and liver tissues of healthy Rattus norvergicus, treated with BPA and BPA with probiotic bacteria Lactobacillus salivarius. The proteins were extracted from the tissues. The isolated proteins were washed with 2-D clean up kit because it is very sensitive to salts and detergents and the protein concentration was quantified for loading the samples in 2-D electrophoresis. Proteins were separated based on IEF and Molecular weight. The gels were Silver stained to observe the protein spots and were scanned by using Epson Expression 11000XL Scanner. We noticed over all 1237 unique proteins in our gels, in that 62 protein spots were isolated. Over all 26 protein spots showed over expression and remaining 36 protein spots showed under expression. Most of the over expressed proteins were identified in Bisphenol treated liver tissue and most of the under expressed proteins were noticed in Bisphenol with probiotic treated hypothalamus. Scattered plots were drawn between the obtained protein match IDs. Further, this study helps to the functional analysis through MALDI-TOF.