20Dec 2016

CLINICOPATHOLOGICAL SIGNIFICANCE OF CANCER STEM CELLS (CD 44 AND CD133) AND INFLAMMATORY MICROENVIRONMENT (TAMS AND MAST CELLS) IN COLORECTAL CANCER: IMMUNOHISTOCHEMICAL STUDY.

  • Department of Pathology, Faculty of Medicine, Cairo University, Egypt.
  • Department of Pathology, Faculty of Medicine, Fayoum University, Egypt.
  • Department of Medical Oncology, Faculty of Medicine, Zagazig University, Egypt.
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Background: Many studies have investigated the role of CSCs and the role of the inflammatory microenvironment in CRC especially their relation to prognosis. Materials and Methods: To examine the prognostic significance of CSCs and the inflammatory microenvironment in CRC, we selected 2 cancer stem cell markers; CD44 and CD133 and 2 inflammatory microenvironment members; TAMs and mast cells. Sixty cases of radical resection specimens of CRC collected as fresh samples and paraffin blocks from archives used for CD133, CD44, CD68 and CD117 immunohistochemistry. Results: There was a statistically significant relation for CD133 expression and the (T) status of the tumor (P=0.012), while CD44 expression was significantly related to both tumor size (P=0.006) and Dukes classification (P=0.019). The positivity of both markers was significantly related to metastasis (P=0.023). The expression of CD68 was statistically significant in relation to the tumor size (P=0.018), the T (P=0.007) and the N (P=0.049) status of the tumor. The expression of CD117 was also significantly related to the T status of the tumor (P=0.011), M status (P=0.003) and to Dukes classification of the tumor (P=0.001). The CRC cases with infiltration of moderate and strong intensity for both markers had a strongly significant relation to the T status (P=0.027), the metastasis (P=0.034) and the presence of lymphovascular invasion (P=0.011).The expression of CD133 was significantly related to that of CD44 (P=0.013) and to that of CD117 (P=0.013) but was not related to the expression of CD68 (P=0.92). CD44 expression was significantly related to CD68 expression (P=0.013) but not related to the expression of CD117 (P=0.137). Conclusion: CSCs (CD133 and CD44) and inflammatory microenvironment (TAMs and mast cells) play a role in CRC progression referring to a possible cross-talk between them which needs further studies for confirmation.


[Lubna O. Abdel-Salam, Hala M. El-hanbuli, Mostafa A. Abousarie and Rasha Haggag. (2016); CLINICOPATHOLOGICAL SIGNIFICANCE OF CANCER STEM CELLS (CD 44 AND CD133) AND INFLAMMATORY MICROENVIRONMENT (TAMS AND MAST CELLS) IN COLORECTAL CANCER: IMMUNOHISTOCHEMICAL STUDY. Int. J. of Adv. Res. 4 (Dec). 1102-1114] (ISSN 2320-5407). www.journalijar.com


Rasha Haggag


DOI:


Article DOI: 10.21474/IJAR01/2498      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2498