Green synthesis of silver nanoparticles using leaf extract of Cinnamomum tamala and its antimicrobial activity against clinical isolates of bacteria and fungi
Mohammad Jalal
, Mohammad Azam Ansari
, Syed Ghazanfar Ali
, Haris M Khan
, Walid Ahmed Hamid Eldaif
, Sulaiman A. Alrumman
- Department of Microbiology, Nanotechnology and Antimicrobial Drug Resistance Laboratory Jawaharlal Nehru Medical College &Hospital, Aligarh Muslim University, Aligarh -202002 Uttar Pradesh, India.
- Department of Medical Laboratories, College of Applied Medical Science, Buraydah College, Saudi Arabia.
- Faculty of Medical Laboratory Sciences, Al-Neelain University, Sudan.
- Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
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Silver nanoparticles (AgNPs) have been synthesized using the aqueous leaf extract of Cinnamomum tamala. The synthesized particles were characterized by UV-Visible Spectroscopy, SEM, TEM, FTIR and EDX. TEM analysis showed the size of the nanoparticles was in the range of 10-15 nm. The antimicrobial activities of green synthesized nanoparticles against different bacterial and fungal stains were assessed by broth dilution and agar well diffusion methods. The MIC values show that the bacterial strains were more susceptible to AgNPs than fungal strains. The topographical and ultrastructural changes caused by AgNPs in Candida albicans were characterized by SEM and TEM, respectively. The electron microscopy analysis shows the attachment of AgNPs on the surface and disruption of the fungal cell membrane and cell wall.
[Mohammad Jalal, Mohammad Azam Ansari, Syed Ghazanfar Ali, Haris M Khan, Walid Ahmed Hamid Eldaif, Sulaiman A. Alrumman (2016); Green synthesis of silver nanoparticles using leaf extract of Cinnamomum tamala and its antimicrobial activity against clinical isolates of bacteria and fungi Int. J. of Adv. Res. 4 (Dec). 428-440] (ISSN 2320-5407). www.journalijar.com
