IN VITRO ANTICANCER ACTIVITY OF METHANOLIC EXTRACT OF ROOTS OF NAREGAMIA ALATA W&A.

The aim of the study was to evaluate the phytochemical and anti-proliferative activity of the methanol extract of the root of N. alata by MTT Assay. For evaluation A-549 (Lung), Hep G2 (Liver) and HeLa (Cervical) cell lines were used. Various concentrations of the methanolic root extract were used for this study. Qualitative phytochemical analysis of root extract showed the presence of tannins, flavonoids, terpenoids, alkaloids and glycosides. Root extract showed less anti-proliferative activity against A-549 cell lines showed 59.56± 0.09% viability at 100µg/ml of extract and IC 50 value was found to be >100 µg/ml. In case of Hep G2 liver human cancer line and HeLa cervical cell line root methanol extract of plant showed 37.70±0.04% and 44.66±0.014% viability compared with the standard doxorubicin were 26.01±0.007% and 34.05±0.14% respectively. The IC 50 values were found to be 45µg/ml and 67.5 µg/ml for root methanol extract against Hep- G2 and HeLa cell lines, compared with the standard drug doxorubicin were 16.25 and 25µg/ml by MTT assay. The extract showed dose dependent anticancer activity. Root extract is more potent against Hep G2 cell lines than A-549 and HeLa cell lines. Hep G2 showed an antiproliferative activity (IC50) at 45µg/ml of crude extract. The results of the present findings were the potential of the selected plant as a resource for the discovery of novel anticancer, agents.