31Dec 2016

ANTIBACTERIAL ACTIVITY OF CERTAIN MEDICINAL PLANT AND THEIR ESSENTIAL OILS ON THE ISOLATED BACTERIA FROM UTI PATIENTS.

  • The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt.
  • Department of Clinical pathology, Faculty of Medicine, Al-Azhar University (Girls Branch), Cairo, Egypt.
  • Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
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In the current study, UTI bacterial isolates were then analyzed to determine their susceptibility profile to 30 antibiotics according to the standard CLSI guide. Among the tested samples, 919 bacteria isolated from urine samples (88.4%) were sensitive to the 30 tested antibiotics; 121 samples (11.6%) recognized as multi-drug resistant bacteria. The results also indicated that the most of urinary tract infection diseases were by Gram negative bacteria (102 isolates; 84.3 %).Additionally, in the present study among 15 plants extracted by boiled-water, ethanol or tested as essential oils, highest antibacterial activity was exhibited by essential oils plant extracts. Among all plant extracts highest IZ values were recorded for Cinnamomum zeylanicum in the range of 46.3 and 28.4mm against UTI bacterial isolates in following order Staphylococcus aureus > Escherichia coli >Pseudomonas aeruginosa >Enterococcus faecalis >Klebsiella pneumoniae. The next higher activity was observed for Thymus vulgaris, Origanum majorana, Syzygium aromaticum, Zingiber officinale, Salvia officinalis and Rosmarinus officinalis with the same inhibition pattern exhibited by Cinnamomum zeylanicum.Moreover, the ultrastructural effect of cinnamon essential oils on Escherichia coli and Staphylococcus aureus were also studied and showed dramatic cellular alterations on TEM electron micrographs with most of effects on bacterial cell wall.GC/MS analysis of essential oils indicated that cinnamon oils had fifteen components, and cinnamaldehyde was the major constituent (72.87%). On the other hand, the main components of marjoram oil were linalool (29.20%), trpenin-4-ol (20.04%) and ?-terpinen (11.89). However, thyme oil had 17 major components includes P-cymen (29.15%), thymol (24.80%) and carvecol (22.69%).

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[Mohamed H. Mourad,Soheir Abdel-Rahman Salih , Mahmoud M. Elaasser, Nesreen A. Safwat and Mostafa Y. Ibrahim. (2016); ANTIBACTERIAL ACTIVITY OF CERTAIN MEDICINAL PLANT AND THEIR ESSENTIAL OILS ON THE ISOLATED BACTERIA FROM UTI PATIENTS. Int. J. of Adv. Res. 4 (Dec). 1510-1530] (ISSN 2320-5407). www.journalijar.com

Mohmed Hosny Mourad
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt

DOI:

Article DOI: 10.21474/IJAR01/2551      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2551

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