01Jan 2017

CONCENTRATION OF CERAMIDE KINASE (CER) AS ASSAY TO MEASURE CELLULAR LEVELS OF CERAMIDE IN SERUM AND TISSUE BY USING ELIZA TECHNIQUE IN BPH, PC, AND HEALTHY MEN IN IRAQ.

  • Department of chemistry and biochemistry Al-Nahrain college of medicine Baghdad. Iraq.
Crossref Cited-by Linking logo

Abstract


Ceramide is subsequently metabolized by ceramide kinase to generate C1P and by ceramidase to generate sphingosine, which is further phosphorylated to S1P by sphingosine kinase. Alternatively again, dephosphorylating of the metabolic derivate also occurs using specific phosphatases, such as C1P phosphatase and S1P phosphatase ceramide can also be produced from sphingosine by ceramide synthasethe dynamic regulation for ceramide generation and metabolism is critical for cellular responses to extracellular stimuli, such as death receptor-mediated (TNF-and Fas), chemotherapeutic agent-mediated using in prostate cancer (zoladex , prostate car , and irradiation-mediated (UV and ?-irradiation) . For tumorigenesis, ceramide acts as a tumor-suppressor lipid, whereas S1P acts as a tumor-promoting lipid in BPH and PC cancer comparing with healthy men.


References


  1. Antoon, J.W.; White, M.D.; Burow, M.E.; Beckman, B.S. Dual inhibition of sphingosine kinase isoforms ablates TNF-induced drug resistance. Oncol. Rep. 2012; 27:1779?1786.
  2. Abuhusain, H.J.; Matin, A.; Qiao, Q.; Shen, H.; Kain, N.; Day, B.W.; Stringer, B.W.; Daniels, B.; Laaksonen, M.A.; Teo, C.; et al. A metabolic shift favouring sphingosine 1-phosphate at the expense of ceramide controls glioblastoma angiogenesis. J. Biol. Chem. 2013; 288:37355?37364.
  3. Anelli, V.; Gault, C.R.; Snider, A.J.; Obeid, L.M. Role of sphingosine kinase-1 in paracrine/transcellular angiogenesis and lymphangiogenesis in vitro. FASEB J. 2010; 24:2727?2738.
  4. Antoon, J.W.; White, M.D.; Meacham, W.D.; Slaughter, E.M.; Muir, S.E.; Elliott, S.; Rhodes, L.V.; Ashe, H.B.; Wiese, T.E.; Smith, C.D.; et al. Antiestrogenic effects of the novel sphingosine kinase-2 inhibitor ABC294640. Endocrinology 2010; 151:5124?5135.
  5. Arduengo, P.M. Sloppy technicians and the progress of science.wordpress.com/2010/03/15/sloppy-technicians Good, N.E. et al. (1966) Hydrogen ion buffers for biological research. Biochemistry. 2010 ; 5: 467?77.
  6. Brizuela, L.; Ader, I.; Mazerolles, C.; Bocquet, M.; Malavaud, B.; Cuvillier, O. First evidence of sphingosine 1-phosphate lyase protein expression and activity downregulation in human neoplasm: Implication for resistance to therapeutics in prostate cancer. Mol. Cancer Ther. 2012; 11: 1841?1851.
  7. Bektas, M.; Allende, M.L.; Lee, B.G.; Chen, W.; Amar, M.J.; Remaley, A.T.; Saba, J.D.; Proia, R.L.Sphingosine 1-phosphate lyase deficiency disrupts lipid homeostasis in liver. J. Biol. Chem. 2010; 285: 10880?10889.
  8. Baran, Y.; Salas, A.; Senkal, C.E.; Gunduz, U.; Bielawski, J.; Obeid, L.M.; Ogretmen, B.Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apoptosis in K562 human chronic myeloid leukemia cells. J. Biol. Chem. 2007; 282:10922?10934.
  9. Br?gger, B.; Sachsenheimer, T.; Wieland, F.; et al. A critical role for ceramide synthase 2 in liver homeostasis: I. alterations in lipid metabolic pathways. J. Biol. Chem. 2010; 285:10902?10910.

Cite This Article as:


[Raghdah Abdulmonaam Razoqi, Raid Jasem Mohamed, and usama al nasiri. (2017); CONCENTRATION OF CERAMIDE KINASE (CER) AS ASSAY TO MEASURE CELLULAR LEVELS OF CERAMIDE IN SERUM AND TISSUE BY USING ELIZA TECHNIQUE IN BPH, PC, AND HEALTHY MEN IN IRAQ. Int. J. of Adv. Res. 5 (1). 261-264] (ISSN 2320-5407). www.journalijar.com


DOI:


Article DOI: 10.21474/IJAR01/2747       DOI URL: http://dx.doi.org/10.21474/IJAR01/2747


Share this article