18Feb 2017

HEPATOPROTECTIVE EFFECT OF NIGELLA SATIVA ON DIABETIC MICE.

  • Department of Biotechnology, L. N. Mithila University, Darbhanga.
  • Research Centre, Mahavir Cancer Sansthan and Research Centre, Patna.
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Diabetes mellitus is a major health problem for the people of the world. Diabetes is a chronic metabolic disorder resulting from a variable interaction of hereditary and environmental factors and it is characterized by abnormal insulin secretion or insulin receptor affecting ? cells of pancreas. Itis associated with a number of chronic complications including nephropathy, neuropathy, retinopathy and cardiovascular diseases. Diabetes mellitus affects a large number of people throughout the world and India also. Experts estimate that diabetic population will grow from 195 to 360 million by 2030 almost 4.5 percentage of the global population. Present study included histological and biochemical parameters of mice. Three groups of mice were prepared for comparative study on control, diabetic, and N. sativa .Diabetic models were prepared in mice by intraperitoneal administration of single dose of alloxan@120mg/kg b.w. Alcoholic extract of Nigella sativa was administered @100 mg/kg b.w/day for four and eight weeks. In diabetic group of mice glucose, creatinine, urea and SGPT were increased. Effective restoration was observed in glucose, SGPT, urea and createnine of N. sativa administered diabetic group of mice. Liver also shows effective restoration in N. sativa administered group of mice. Thus, it is concluded from study that alcoholic extract of N.sativa restores glucose level to normal. Nigella sativa acts effectively on diabetes mice on biochemical and histological parameters.

  1. World Health Organization: Global Health Risks, Mortality and Burden of Disease Attributable to Selected Major Risks. December: 2010.
  2. Wild S., Roglic G., Green A., Sicree R. and King H; Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care:2004; 27(5): 1047–53.
  3. King H., Auburt RE. and Herman WH; Diabetes Care: 1998; 21:1414- 31.
  4. Ramachandran A., Socioeconomic burden of diabetes in India. Suppl. JAPI: 2007; 55:9.
  5. Kota S.K., Meher L.K., JammulaS, Kota S.K. and Modi K.D; Genetics of type 2 diabetes mellitus and other specific types of diabetes; its role in treatment modalities. DiabetesMetab. Syndr: 2012; 6, 54–58.
  6. Silva, Y.T.C., Peres L.C. and Foss M.C; Enamel hypoplasia in a litter of rats with alloxan- induced diabetes mellitus. Braz. Dent. J: 2003;14(2):65-69.
  7. Astrup AS., Cardiovascular morbidity and mortality in diabetes mellitus prediction and prognosis. Dan Med Bull: 2011; 58(8):B4152.
  8. Garodia P., Ichikawa H.,Malani N., Sethi G. and Aggarwal BB;from ancient medicineto modern medicine: ayurvedic concepts of health and their role in inflammation and cancer. J SocIntegrOncol: 2007;5:25-37.
  9. Elshiekh Y.H.AndMona A M abdelmageed; Phytochemical screening and antimicrobial activity of Strigahermonthica and Nigella sativa seeds. American Journal of Research Communication: 2015; 3(3): 24-33.
  10. El Tahir KE.,AshourMM.andal-Harbi MM; The cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: elucidation of the mechanism of action. Gen Pharmacol: 1993; 24(5):1123-31.
  11. , J., S.A. Ahmed., S.P. Akpulu., G.K. Owolagba. and M.U. Iduh; Protective effect of cool extraction of black seed (Nigella sativa) oil against CCl4-induced oxidative damages in Wistar Rats testis. IOSR J. Pharmacy Biological Sci: 2013; 5: 68-74.
  12. Kanter M., Coskun O. and Budancamanak M; Hepatoprotective effects of Nigella sativa and UrticadioicaL. on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. W. J. Gastroenterol: 2005A; 11: 6684–6688.
  13. Zaidi SF., Yamada K., Kadowaki M., UsmanghaniK.and Sugiyama T; Bactericidal activity of medicinal plants, employed for the treatment of gastrointestinal ailments, against Helicobacter pylori. J Ethnopharmacol: 2009;121: 286-91.
  14. Krishnamurthy, B., J. Chee, G. Jhala, S. Fynch and K.L. Graham et al., 2012. Complete diabetes protection despite delayed thymic tolerance in NOD8.3 TCR transgenic mice due to antigen-induced extrathymic deletion of T Cells. Diabetes, 61: 425-435.
  15. Armas LA, Akhter MP, Drincic A and Recker RR. Trabecular bone histomorphometry in humans with Type 1 Diabetes Mellitus. Bone 2012; 50: 91-96.
  16. Levey AS, Bosch JP, Lewis JB, Greene T, Rogers N, Roth D. A more accurate method to estimate glomerular filtration rate from serum creatinine: a new prediction equation. Modification of Diet in Renal Disease Study Group. Ann Intern Med 1999; 130: 461-470
  17. Kanter M., Coskun O. and Budancamanak M; Hepatoprotective effects of Nigella sativa and UrticadioicaL. on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. W. J. Gastroenterol: 2005A; 11: 6684–6688.
  18. Gani MS, John SA. Evaluation of hepatoprotective effect of Nigella Sativa Int J Pharm PharmSci 2013;5:428-30.
  19. El-Gharieb MA, El-Masry TA, Emara AM, HashemMA. Potential hepatoprotective effects of vitamin E and Nigella sativa oil on hepatotoxicity induced by chronic exposure to malathion in human and male albino rats. Toxicol Environ Chem 2010;92:391-40.
  20. Hassan AS, Ahmed JH, Al-Haroon SS. A study of the effect of Nigella sativa (Black seeds) in isoniazid (INH)-induced hepatotoxicity in rabbits. Indian J Pharmacol 2012;44:678-82.
  21. Mansour MA. Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice. Life Sci 2000;66(26):2583-91.

[Sneha Navin, Jaykar Jha, Abhinav, Arun Kumar, Mohammad Ali, Ashok Ghosh and Ranjit Kumar. (2017); HEPATOPROTECTIVE EFFECT OF NIGELLA SATIVA ON DIABETIC MICE. Int. J. of Adv. Res. 5 (Feb). 181-188] (ISSN 2320-5407). www.journalijar.com

Dr Ranjit Kumar
Mahavir Cancer Institute and Research Centre, Patna, Bihar, India

DOI:

Article DOI: 10.21474/IJAR01/3130      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3130

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