18Feb 2017

CYTOGENETICS AND MOLECULAR GENETICS ON FEMALE INFERTILITY WITH SPECIAL EMPHASIS ON POLYCYSTIC OVARIAN SYNDROME

  • Department of Zoology, Mar Ivanios College, Thiruvananthapuram, Kerala.
  • Research Scholar, Meenakshi University, West K K Nagar, Chennai, Tamil Nadu.
  • Department of Biochemistry, Jubilee Mission Medical College and Research Institu te, Thrissur, Kerala.
  • Genetika, Centre for Advanced Genetic Studies, Pettah PO, Thiruvananthapuram, Kerala.
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Female infertility is defined as the inability to conceive naturally or to carry a pregnancy to full term. It occurs for almost 15% of all women worldwide. The etiology of infertility is an important criterion for recognition and characterization of infertile women. The common factors for infertility in females are anovulatory disorder, tubal factors, endometriosis, uterine and cervical factors. Infertility affects 40% of women with PCOS and is the most common cause of female infertility. The present study was undertaken to evaluate the cytogenetics and molecular genetics on female infertility with special emphasis on polycystic ovarian syndrome by investigating the various anthropometric and clinical aspects of the subjects. Seventy five female subjects with infertility and 50 healthy women without any chronic illness were involved in this study. Reproductive hormones namely leutinizing hormone (LH), follicle stimulating hormone (FSH), prolactin and estradiol were estimated in each subjects after obtaining their informed consent. Cytokinesis-block micronuclei (CBMN) assay was also carried out in the lymphocytes of the subjects to assess the somatic DNA damage. The study demonstrated that the micronuclei frequency significantly elevated in the study subjects than control subjects. Infertile women with various risk factors such as increasing age, BMI, family history of infertility, family history of cancer, menarche, endometriosis etc. can lead to increased genetic instabilities and the severity of infertility. Lifestyle modification with diet and exercise will reduce the risk for infertility.

  1. Azziz, R,. Woods, K.S., Reyna, R., Key, T.J., Knochenhauer, E.S., Yildiz, B.O. (2004): The prevalence and features of the polycystic ovary syndrome in an unselected population. J ClinEndocrinol Metab., 89: 2745?2749.
  2. Boomsma, C.M., Eijkemans, M.J., Hughes, E.G., Visser, G,H., Fauser, B.C., Macklon, N.S. (2006): A meta-analysis of pregnancy outcomes in women with polycystic ovary syndrome. Hum Reprod Update., 12: 673-83.
  3. Gaware, V.M., Parjane, S.K., Merekar, Abhijit, N., et al., (2009): Female infertility and its treatment by alternative medicine: a review. J Chem Pharm Res., 1: 148?162.
  4. Gekas, J., Thepot, F., Turleau, C., et al., (2001): Chromosomal factors on infertility in candidate couples for ICSI: an equal risk of constitutional aberrations in women and men. Hum Reprod., 16: 82-90.
  5. Matzuk, M.M., Lamb, D.J. (2008): The biology of infertility: research advances and clinical challenges. Nat Med., 14: 1197 ? 1213.
  6. Norman, R.J., Davies, M.J., Lord, J. and Moran, L.J. (2002): Role of lifestyle modification in polycystic ovary syndrome. Trends Endocrinol Metab., 13: 251-257.
  7. W.E., Amballi, A.A., Banjo, T.A. (2012): A review of female infertility important etiological factors and management. J Microbiol Biotech Res., 2(3): 379?385.
  8. Sanderson, M.L., Hassold, T.J., Carrell, D.T., (2008): Proteins involved in meiotic recombination: a role in male infertility? SystBiolReprod Med., 54: 57? 74.
  9. The ESHRE Capri Workshop Group. (2008): Genetic aspects of female reproduction. Hum Reprod Update., 14: 293 ? 307.
  10. Unuane, D., Tournaye, H., Velkeniers, B., Poppe, K. (2011): Endocrine disorders & female infertility. Best Pract Res ClinEndocrinolMetab., 25(6): 861-873.
  11. Guastella, E., Longo, R.A., Carmina, E. (2010): Clinical and endocrine characteristics of the main polycystic ovary syndrome phenotypes. Fertil Steril., 94(6): 2197-2201.
  12. Wijeyaratne, C.N., Jayasinghe, A., de Silva, D.G., Parkes, A.B., Lazarus, J.H., Premawardhana, L.D. (2005): Iodine prophylaxis, goitre and thyroid autoimmunity in Sri Lanka Ceylon Med J. Mar., 50(1): 20-3.
  13. Ewens, K.G., Jones, M.R., Ankener, et al., (2011): FTO and MC4R gene variants are associated with obesity in polycystic ovary syndrome, PLoS One.,6: Article ID e16390.
  14. Mokhtar, S., Hassan, H.A., Mahdy, N., Elkhwsky, F., Shehata, G. (2006): Risk factors for primary andsecondary female infertility in Alexandria: ahospital-based case-control study. JMRI., 27: 255?261.
  15. Zlotogora, J., Hujera,t Y,, Barges, S., Shalev, S.A., Chakravarti, A. (2006): The fate of 12 recessive mutations in a single village. Ann Hum Genet., 202?208.
  16. Moghadam, D.A., Delpisheh, A. and Khosravi, A. (2013): Epidemiology of Female Infertility; A Review of Literature. Biosciences Biotechnology Research Asia., 559-567.
  17. Maeda, K., Tsukamura, H. (2006): The Impact of Stress on Reproduction: are Glucocorticoids Inhibitory or Protective to Gonadotropin Secreation? Endocrinology., 147: 1085-6.
  18. Jain, V., Saha, S.C., Bagga, R, Gopalan S. Unsafe abortion: a neglected tragedy. J Obstet Gynaecol Res. 2004; 30: 197-201.
  19. World Health Organization. (2003): Safe Abortion: Technical Policy Guidance for Health System., 13-4.
  20. ESHRE Capri workshop group (2000): Optimal use of infertility diagnostic tests and treatments. Hum Reprod. 15,723?732.
  21. Broekmans, F.J., Knauff, E.A., te Velde, E.R., Macklon, N.S., Fauser, B.C. (2007). Female reproductive ageing: current knowledge and future trends. Trends Endocrinol Metab., 18: 58 ? 65.
  22. Liehr, T., Claussen, U., Starke, H. (2004). Small supernumerary marker chromosomes (sSMC) in humans. Cytogenet Genome Res., 107: 55-67.
  23. Shah, K., Sivapalan, G., Gibbons, N., Tempest, H,, Griffin, D.K. (2003). The genetic basis of infertility. Reproduction; 126:13-25.
  24. Diamanti-Kandarakis. E., Kouli, C., Tsianateli, T., Bergiele, A. (1998). Therapeutic effects of metformin on insulin resistance and hyperandrogenism in polycystic ovary syndrome. Eur J Endocrinol., 138: 269?274.
  25. Svendsen, P.F., Nilas, L., Norgaard, K., Madsbad, S. (2005). Polycystic ovary syndrome. New pathophysiological discoveries therapeutic consequences. Ugeskr Laeger., 22;167(34): 3147-51.

[Josey Ann Jijo, Arun William, Aneesh P, Leenamma Joseph, Lija L Raju, Viji Krishnan and Dinesh Roy D. (2017); CYTOGENETICS AND MOLECULAR GENETICS ON FEMALE INFERTILITY WITH SPECIAL EMPHASIS ON POLYCYSTIC OVARIAN SYNDROME Int. J. of Adv. Res. 5 (Feb). 483-488] (ISSN 2320-5407). www.journalijar.com

Dr. Dinesh Roy D
Genetika, Centre for Advanced Genetic Studies, Pettah PO, Thiruvananthapuram, Kerala

DOI:

Article DOI: 10.21474/IJAR01/3175      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3175

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