20Mar 2017

IN VITRO PROPAGATION OF SPATHOGLOTTIS PLICATA BLUME VIA ASYMBIOTIC SEED GERMINATION.

  • Department of Biotechnology, SAFI Institute of Advanced Study, Malappuram 673633, India.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The loss of plant genetic resources has necessitated the development of many ex situ conservation techniques. The application of plant tissue culture techniques in orchid conservation and propagation requires an efficient in vitro regeneration protocol. This study reports the development of such highly efficient protocols for the in vitro asymbiotic seed germination of Spathoglottis plicata. The seeds were successfully germinated asymbiotically on Gamborg B5 (1968).Various growth regulators such as 2,4-Dichlorophenoxyacetic acid (2,4-D), Indole acetic acid (IAA) Benzyladenine (BA) and Kinetin individually were used for callus induction and multiple shoot initiation from the protocorms. B5 medium supplemented with 2,4-D (9.03 ?M) was suitable for callus induction. Calli developed a route of production of protocorm-like bodies and eventually develop into plantlets on transfer to growth regulator free half strength basal medium. The well rooted plants were hardened successfully in the potting mixture containing coconut husk, sand, charcoal, and brick pieces in the ratio 1:1:1:1.


  1. Arditti, J. and Ernst, R. (1984). Physiology of germinating orchid seeds. Cornell University Press, Ithaca, 177-222
  2. Arditti, J. and Ernst, R. (1990). Micropropagation of Orchids. 1st edition, John Wiley and Sons, New York.
  3. Beltrame, E. (2006). Spathoglottis-Inside and Out. Orchid Review, 114: 68-71
  4. Chou, L.C. and Chang, D.C.N. (2004). Asymbiotic and symbiotic seed germination of Anoectochilus formosanus and Haemaria discolor and their F1 hybirds. Bot. Bull. Acad. Sin, 45: 143?147
  5. Debergh, P.C. and Zimmerman, R.H. (1991). Micropropagation: technology and application. Kluwer Academic Publishers, Dordrecht, The Netherlands
  6. Dressler, R.L. (1993). Phylogeny and Classification ofthe Orchid Family, Cambridge University Press, UK, 314 pp.
  7. Hagsater, E. and Dumont, V. (1996). Orchids: Status Survey and Conservation Action Plan. IUCN Publications Services Unit, U.K
  8. Ishii, Y. Takamura, T. Goi, M. and Tanaka, M. (1998). Callus induction and somatic embryogenesis of Phalaenopsis, Plant Cell Reports, 17: 446-450.
  9. Jain, S.K. (1980). Orchid and mountain flora of India.67th Session Indian Sci. Conger. Assoc, Calcutta
  10. Johnson, T.R. Stewart, S.L. Daniela, D. Kane, M. E. and Richardson, L. (2007). Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae)?preliminary evidence ????????? for the symbiotic culture advantage. Plant Cell Tissue Organ Cult. 90: 313?323.
  11. Kasulo, V. Mwabumba, L. and Cry, M. (2009). A review of edible orchids in Malawi. Journal of Horticulture and Forestry. 1 (7): 133?139.
  12. Kauth, P.J. Wagner, A. Vendrame. and Kane, M.E. (2006). In vitro seed culture and seedling development of Calopogon tuberosus. Plant Cell, Tissue and Organ Culture, 85: 91?102.
  13. Kheawwongjun, J. and Thammasiri, K. (2008). Breeding Spathoglottis spp for commercial potted orchids. Proceedings of the International Workshop on Ornamental Plants. Eds Chomchalow N & Chantasmi V. Acta Horticulturae 788
  14. Lang, N.T. and Hang, N.T. (2006). Using biotechnological approaches for Vanda orchid improvement, Omonrice, 14: 140-143
  15. Lee, Y. and Lee, N. (2003). Plant regeneration from proto-corm-derived callus of Cypripedium Formosanmun, In Vitro Cellular and Developmental Biology?Plant, 39(5): 475-479.
  16. Lin,Y.H., C. Chang & W.C. Chang, 2000. Plant regeneration from callus culture of a Paphiopedilum hybrid, Plant Cell Tissue and Organ Culture, 62: 21?25.
  17. Malmgren, S. (1992). Large scale asymbiotic propagation of Cypripedium calceolus?plant physiology from a surgeon?s point of view. Royal Botanic Gardens Kew. Micropropagation News, 15: 59?63.
  18. Naing, A.H. Chung, J.D. and Lim, K.B. (2011A). Plant regeneration through indirect somatic embryogenesis in Coelogyne cristata orchid. American Journal of Plant Sciences, 2: 262-267.
  19. Naing, A.H. Chung, J.D. Park, I.S. and Lim K.B. (2011B). Efficient plant regeneration of the endangered medicinal orchid, Coelogyne cristata using protocorm-like bodies. Acta Physiologiae Plantarum, 33: 659?666.
  20. Pant, B. and Raskoti, B.B. (2013). Medicinal orchids of Nepal. Kathmandu, Nepal: Himalayan Map House (P.) Ltd.104p
  21. Spoerl, E. and Curtis, J.T. (1948). Studies on the nitrogen nutrition of orchid embryos. III. Amino acid nitrogen. Amer. Orchid Soc. Bull., 17: 307-312
  22. Stewart, S.L. and Kane M.E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Organ Cult.86: 147?158.
  23. Subedi, A. Kunwar, B. Choi, Y. Dai, Y. Andel, T.V. Chaudhary, R.P. Boer, H.J and Gravendeel, B. (2013). Collection and trade of wild-harvested orchids in Nepal. Journal of Ethnobiology and Ethnomedicine, 10: 9-64.
   

[Aswathi, Sahaya Shibu, Agila Gopinath and Akhila Mohan. (2017); IN VITRO PROPAGATION OF SPATHOGLOTTIS PLICATA BLUME VIA ASYMBIOTIC SEED GERMINATION. Int. J. of Adv. Res. 5 (Mar). 431-438] (ISSN 2320-5407). www.journalijar.com


Aswathi, Sahaya Shibu
Department of Biotechnology, SAFI Institute of Advanced Study, Malappuram 673633, India

DOI:


Article DOI: 10.21474/IJAR01/3530      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3530