22Apr 2017

ANALYSIS OF GENETIC STABILITY OF THE IN VITRO PROPAGATED ROSA DAMASCENA (TAIF ROSE) PLANT.

  • Biotechnology and Genetic Engineering Research Unit, Deanship of Scientific Research, Taif University, Taif, Al-Haweiah, P.O. Box 888, Zip code 21974, Taif, KSA.
  • Agricultural Genetic Engineering Research Institute, Agricultural Research Center, P.O. Box, 12619, Giza, Egypt.
  • Faculty of Science, Arish University, Al-Arish, Egypt.
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Through optimization processes of plants by mass production using micropropagation or using genetic transformation, the most important aspect is to retain genetic integrity with reference to the mother plants. It is important to retain and improve the Taif rose (Rosa damascena) plant for its economic and medical importance. Although no or minor morphological variations were recorded in some micropropagated plants. In present study, Randomly Amplified Polymorphic DNA (RAPD) markers were employed to determine the genetic fidelity of Taif rose plantlets multiplied in vitro by using nodal segments. Among the screened 10 RAPD primers, were generated total of 801 clear, distinct and reproducible bands. Among these bands, 798 bands were monomorphic (99.63 %) and 3 bands were polymorphic (0.37 %). This very low polymorphism ratio between mother plants and micropropagated plantlets indicates the little effect of somaclonal variations and the high genetic similarity between mother plants and micropropagated plants. The molecular profiling by using RAPD markers proved to be an effective method and can be applied for assessing genetic stability of micropropagated plants. Thus, the developed in vitro plantlets which ascertained stability can be used successfully.


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[Hossam M. Zakaria, Eldessoky S. Dessoky, Ismail A. Ismail, Attia O. Attia and Ehab I. El-Hallous. (2017); ANALYSIS OF GENETIC STABILITY OF THE IN VITRO PROPAGATED ROSA DAMASCENA (TAIF ROSE) PLANT. Int. J. of Adv. Res. 5 (Apr). 117-122] (ISSN 2320-5407). www.journalijar.com


Hossam Mohammed Zakaria
Agricultural Genetic Engineering Research Institute, Agricultural Research Center, P.O. Box, 12619, Giza, Egypt.

DOI:


Article DOI: 10.21474/IJAR01/3787      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3787