22Apr 2017

LEVERAGE OF SYNTHETIC VITAMINS (C AND E) ON ANTIOXIDANT ACTIVITY IN BROILERS CHALLENGED WITH E. COLI.

  • Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia, Egypt 41522.
  • Department of Animal Hygiene, Zoonosis & Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt 41522.
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Vitamin E as a lipid component of biological membranes is known to be a major chain-breaking antioxidant and vitamin C or ascorbic acid is an anti-oxidant which is normally synthesized by the chicken. A comparative study was carried out to demonstrate the influence of synthesized vitamin C and vitamin E on bird performance, biochemical, antioxidant and immunological status. 150 broiler chick was received from day one age; divided into three groups; G1 (supplemented with synthetized vitamin C 3.5 g / 1 L water); G2 (supplemented with synthetized vitamin E 1 mL / 1 L water) and G3 (Control). G1 and G2 were challenged at 2nd week of age with E. coli O157:H7 1.3 X 106 CFU. A total of 180 samples (60 serum, 60 plasma and 60 intestinal swab) were collected during the study period. The results revealed that both synthetized vitamin E and vitamin C were able to enforce a protective effect in the birds last for only 4 week then vanished gradually. Synthetized vitamin E was superior to the vitamin C in enforcing a good protective effect on the birds as vitamin E showed more prominent stimulation of immunoglobulin (IgG; IgM. IgE) and bacterial counts were reduced significantly compared to the influence of vitamin C. The study concluded the necessity of the treatment continuation in order to maintain the enhancing effect on antioxidant enzymatic activity and protective immunological effect.

  1. Agius, F., Gonz?lez-Lamothe, R., Caballero, J.L., Mu?oz-Blanco, J., Botella, M.A., Valpuesta, V. (2003). Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase. Nature Biotechnology 2003, 21(2): 177-181.
  2. Altan, O., Pabuccuoglu, A., Altan, A., Konyalioglu, S., Bayraktar, H. (2003). Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. Br. Poult. Sci. 44(4):545?550.
  3. Ayo, J.O., Oladele, S.B., Fayomi, A. (1996). Effect of heat stress on livestock production: A review. Nigerian Veterinary Journal 58-68, Special edition, 1(1): 58-68.
  4. Bautista-Ortega, J., Cortes-Cuevas, A., Ellis, E.A., Ruiz-Feria, C.A. (2014). Supplemental L-arginine and vitamins E and C preserve xanthine oxidase activity in the lung of broiler chickens grown under hypobaric hypoxia. Sci.;93(4):979-988
  5. Berne, G.H. (1974). Clin. Chem. 200, 61-89.
  6. Bottje, W.G., Wang, S., Kelly, F.J., Dunster, C., Williams, A., Mudway, I. (1998). Antioxidant defenses in lung lining fluid of broilers: Impact of poor ventilation conditions. Poult. Sci. 77(4):516?522.
  7. Cruickshank, R., Duguid, J.P., Marimion, B.P., Swain, R.H. (1975). Medical Microbiology, the Practice of Medical Microbiology. 12th Ed, Vol. 11, Churchill Livingstone Limited, Edinburgh, London and New York.
  8. Cruickshank, R., Duguid, J.P., Marimion, B.P., Swain, R.H. (1980). Medical Microbiology. E.L.B.S., 12th Ed, Vol. 11, Reprinted Churchill Livingstone and Robert Stevenson, Edinburgh, EHI, 3AF.
  9. Davey, M.W., Montagu, M.V., Inze, D., Sanmartin, M., Kanellis, A., Smirnoff, N., Benzie, I.J.J., Strain, J.J., Favell, D., Fletcher, J. (2000). Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. Sci. Food Agric., 80, 825-860.
  10. Ghazi, S., Amjadian, T., Norouzi, S. (2015). Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition. J. Biometeorol.; 59(8):1019-24.
  11. Goldberg, D.M., Spooner, R.J. (1983). Methods of enzymatic analysis (Bergmeyen, HV Ed.) 3rd Vol 3, 258-265, verlog chemie, Deerfield beach, Fl.
  12. Halliwell, B., Gutteridge, J.M.C. (2000). Free Radicals in Biology and Medicine. 3rd ed Oxford Univ. Press; New York, NY, USA.
  13. Heremans, J.P., Masson, P.L. (1973). Clin. Chem. 19:294-300.
  14. Herigstad, B., Hamilton, M., Heersink, J. (2001). How to optimize the drop plate method for enumerating bacteria. J. Microbiol. Meth. 44(2):121-129.
  15. Herrera, E., Barbas, C. (2001). Vitamin E: Action, metabolism and perspectives. J. Physiol. Biochem. 57:43-56.
  16. Horning, D. (1975). Ann. NY. Acad. Sci. 258: 103.
  17. Hu, Z.P.,?Wang, T.,?Ahmad, H.,?Zhang, J.F.,?Zhang, L.L.,? Zhong, X. (2015). Effects of different formulations of α-tocopherol acetate (vitamin E) on growth performance, meat quality and antioxidant capacity in broiler chickens. Br Poult. Sci.; 56(6):687-695.
  18. Kaiser, M.G., Block, S.S., Ciraci, C., Fang, W., Sifri, M., Lamont, S.J. (2012). Effects of dietary vitamin E type and level on lipopolysaccharide-induced cytokine mRNA expression in broiler chicks. Poult. Sci. 91(8):1893?1898.
  19. Lauridsen, C., Engel, H., Jensen, S.K., Craig, A.M., Traber, M.G. (2002). Lactating sows and suckling piglets preferentially incorporate RRR-over all-rac-α-tocopherol into milk, plasma and tissues. J. Nutr. 132(6):1258?1264.
  20. Leshchinsky, T.V., Klasing, K.C. (2001). Relationship between the level of dietary vitamin E and the immune response of broiler chickens. Poult. Sci. 80:1590-1599.
  21. Levesque, (2007). SPSS Programming and Data Management: A Guide for SPSS and SAS Users, Fourth Edition (2007), SPSS Inc., Chicago Ill.
  22. Mezes, M., Surai, P., Salyi, G., Speake, B.K., Gaal, T., Maljian, A. (1997). Nutritional metabolic diseases of poultry and disorders of the biological antioxidant defence system. Acta Vet. Hung. 45:349-360.
  23. Nishikimi, M., Roa, N.A., Yogi, K. (1972). Biochem. Bioph. Res. Coomon., 46: 849-854.
  24. Nolan, J., Hinch, G., Twaite, S.J., Walkden-Brown, S. (1999). Constrains to animal production, Chapter 2: Climatic constrains, Lecturer Paper 12, Animal Science Group Publisher, Australia.
  25. Оdinokov, V.N., Spivаk, A.Y., Еmеlyanоvа, G.A., Маllyabаеvа, M.I., Nazarova, O.V., Dzhemilev, UM. (2003). Synthesis of α-tocopherol (vitamin E), vitamin K1-chromanol, and their analogs in the presence of aluminosilicate catalysts Tseokar-10 and Pentasil. ARKIVOC (xiii): 101-118.
  26. Paglia, D.E., Valentine, W.N. (1967). J. Lab. Clin. Med. 70: 158-169.
  27. Rafiee, F., Mazhari, M., Ghoreishi, M., Esmaeilipour, O. (2016). Effect of lemon verbena powder and vitamin C on performance and immunity of heat-stressed broilers. Anim. Physiol. Anim. Nutr. doi: 10.1111/jpn.12457.
  28. Rey, A.I., Segura, J., Olivares, A., Cerisuelo, A., Pi?eiro, C., L?pez-Bote, C.J. (2015). Effect of micellized natural (D-α-tocopherol) vs. synthetic (DL-α-tocopheryl acetate) vitamin E supplementation given to turkeys on oxidative status and breast meat quality characteristics. Poult. Sci.; 94(6):1259?1269.
  29. Rice, D.A., Y.S. Kennedy. (1988). Vitamin E and free radical formation. Pages 39?57 in Recent Advances in Animal Nutrition. W. Haresign and D.J.A. Cole, ed. Butterworths, London, UK.
  30. Sahin, K., Sahin, N., Sari, M., Gursu, M.F. (2002). Effects of vitamins E and A supplementation on lipid peroxidation and concentration of some mineral in broilers reared under heat stress (32?C). Nutr. Res. 22:723?731.
  31. Tripathi, R.P., Singh, B., Bisht, S.S., Pandey, J. (2009). L-Ascorbic acid in Organic Synthesis: An Overview. Current Organic Chemistry 13: 99-122.
  32. Voljč, M., Frankič, T., Levart, A., Nemec, M., Salobir, J. (2011). Evaluation of different vitamin E recommendations and bioactivity of α-tocopherol isomers in broiler nutrition by measuring oxidative stress in vivo and the oxidative stability of meat. Poult. Sci. 90(7):1478?1488.
  33. Young, D.S. (1995). Effects of drugs on clinical lab. Tests, 4th ed, AACC Press.
  34. Young, D.S. (2001). Effects of disease on clinical lab. Tests, 4th ed, AACC Press.
  35. Zelver, N., Hamilton, M., Pitts, B., Goeres, D., Walker, D., Sturman, P., Heersink, J. (1999). Measuring antimicrobial effects on biofilm bacteria: in R. J. Doyle, et al. (eds), biofilm: methods in enzymology. Academic Press, San Diego, CA, pp. 608-628.

[Rasha F. Muhammad, Aida H. Soliman and Essam S. Soliman. (2017); LEVERAGE OF SYNTHETIC VITAMINS (C AND E) ON ANTIOXIDANT ACTIVITY IN BROILERS CHALLENGED WITH E. COLI. Int. J. of Adv. Res. 5 (Apr). 182-188] (ISSN 2320-5407). www.journalijar.com

Essam S. Soliman
Department of Animal Hygiene, Zoonoses and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

DOI:

Article DOI: 10.21474/IJAR01/3794      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3794

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