Study on protection role of BCG Vaccine in Immunization against Enteropathogenic Escherichia coli and there challenge Infection and Pathology in white albino mice

Abstract: This study aimed to determine the role of BCG in immunization of white mice against EnteropathogenicEscherichia coli (EPEC) and there role against challenge infection and pathology with this microbial agent . EPEC was isolated from diarrhea ofchildren , routinely diagnosed and serotyping were identified as 0119 EPEC . Then three groups of mice (30 each group) were taken , first group immunized with whole killed EPEC Ag, the second group were immunized with whole killed EPEC AgandBCG Vaccine , the immunization were occurred at two doses 14 days intervals , third group were injected with phosphate buffer saline ( pbs- control group).Results revealed at 28th days post immunization , increase in DTH- skin test thickness at 24, 48 hrs in group immunized with whole killed EPEC Ag and BCG ( group2 ) comparable to group 1 ( immunized with whole killed cell Ag alone and group 3 ( control group) . Also humeral immune (HI) response elevated in group 2 comparable to group 1 and control group3 . The HI response accompanied by elevation of IgG and IgM level in group 2 comparable to group 1 and control group 3 . On another hand bacterial isolation were mild in group 2 and moderate in group 1 compable to control group 3 following challenge dose infection with EPEC. Also more localized granulomatous lesion were seen in internal organs specially at 14th. , 21th days post inoculation of challenge dose in group 2 comparable to group 1 and no lesion in control group 3. Conclusion : 1- Immunization group 2 of white mice with whole killed EPEC Ag and BCG vaccine give high level of CMI and HI with elevation of IgG, IgM comparable to group 1 immunized with whole killed EPEC Ag alone . 2- Mild bacterial isolates seen in internal organs of immunized group 2 with whole killed EPEC Ag and BCG vaccine comparable to moderate bacterial isolates in whole killed EPEC Ag alone group 1 . 3- More localized granulomas in internal organs of immunized group 2 with whole killed EPEC and BCG vaccinecomparable to mild granulomas in immunized whole killed EPEC Ag alone group 1.

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[Dr. Khalil H.Al-Jeboori and Marwa S. Kezar (2014); Study on protection role of BCG Vaccine in Immunization against Enteropathogenic Escherichia coli and there challenge Infection and Pathology in white albino mice Int. J. of Adv. Res. 2 (4). 0] (ISSN 2320-5407). www.journalijar.com

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