MOLECULAR CHARACTERIZATION OF KEROSENE DEGRADING BACTERIA ISOLATED FROM KEROSENE POLLUTED SOIL

Bacillus thuringiensis was identified has high potential degradation of kerosene. The organism was identified from the kerosene polluted soil sample. 8 different colonies were isolated from the contaminated soil. Colonies were subjected to morphological and biochemical characterization. Then the bacterial isolated were inoculated in the Bushnell Hass medium with 1% of Kerosene which acts as the sole carbon source for their growth. KC1, KC6 and KC8 different isolates were identified which utilizes the Kerosene and grows. Then the isolates were studied for their degradation by turbidometric method. Antibiotic sensitivity test showed all the isolates KC1 to KC8 were Ampicillin resistant, sensitive to Chloramphenicol, Kanamycin and Gentamycin and KC7 was resistant to streptomycin. By spectrometric method the KC1 isolate has high degrading potential of kerosene. The potential organism can be used further for removing of the contamination from the polluted area.