28May 2017

ISOLATION AND IDENTIFICATION OF CHLORPYRIFOS DEGRADING BACTERIA FROM AGRICULTURAL SOIL.

  • Assistant Professor, Department of Microbiology, Jaya College of Arts and Science, Thiruninravur and Research Scholar, PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, Tamilnadu, India.
  • Head and Research Coordinator, PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, Tamilnadu, India.
  • Post Graduate Scholar, PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, Tamilnadu, India.
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The extensive use of pesticides in agricultural fields for pest control pose a serious problem in contaminating soil and water ecosystems. Chlorpyrifos is the major broad spectrum organophosphorus insecticide used in paddy fields against sucking, chewing, boring insects. Due to its toxicity and persistence in the environment, there is an immediate need to eliminate them from contaminated sites by biodegradation. Two pesticide degrading bacteria were screened and isolated from chlorpyrifos contaminated soil by enrichment culture technique and were identified as Kocuria kristinae and Staphylococcus aureus. The growth response and degradation of chlorpyrifos by the isolates in MSM broth supplemented with 0.5% chlorpyrifos was monitored every 48-72 hrs in spectrophotometer at 600nm. Kocuria sp showed maximum growth in 7 days than Staphylococcus aureus. The degradation efficiency of the strains were determined and estimated by the removal percentage of chlorpyrifos from the liquid culture. Both the isolate showed the degrading capability of chlorpyrifos in MSM. The isolate, S. aureus was more potent in degrading the 80% of the total compound from the media in 2 weeks of incubation than K. kristinae which shows 35 % of degradation. These results were further confirmed by GCMS, in which S. aureus has degraded 82.06% and K. kristinae has degraded 30.78% of chlorpyrifos in the medium. This study indicates that the isolate, Staphylococcus aureus is more potent in degrading chlorpyrifos in liquid culture and can also be used in bioremediation of chlorpyrifos contaminated soils.

  1. K. Singh and A. Walker. (2006). Microbial degradation of organophosphorus compounds. FEMS Microbiol Rev. 30, 428?471.
  2. A. Y. Sorgob, E. Vilanova. (2002). Enzymes involved in the detoxification of organophosphorus, carbamate and pyrethroid insecticides through hydrolysis. Toxicol. Lett. 128, 215?228.
  3. Rani, L. K. Surekha, D. P. Vijaya, M. J. R. Suvarnalatha, J. S. K. Aruna, and G. Narasimha. (2008). Venkateswarlu. Isolation and characterization of a chlorpyrifos degrading bacterium from agricultural soil and its growth response. African J Microbiol Res 2, 026?031.
  4. J. Parmar, R. S. Tomar, M. V. Parakhia, B. J. Malviya, V. M. Rathod, J. R. Thakkar, V. V. Kothari, A. J. Bhatt, R. L. Bhalara, and B. A. Golakiya. (2014). Isolation and bio-analytical characterization of chlorpyrifos degrading bacteria. J Cell Tissue Res. 14, 4641?4646.
  5. Chishti and M. Arshad. (2012). Growth linked biodegradation of chlorpyrifos by Agrobacterium and Enterobacter sp. Int J Agric Biol, 15, 19?26.
  6. K. Singh, A. Walker, J. A. W. Morgan and D. J. Wright. (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl Environ Microbiol, 70, 4855?4863.
  7. Barathidasan, and D. Reetha. (2013). Biodegradation of chlorpyrifos and their intermediate metabolites identified by liquid chromatography mass spectroscopy (LC-MS). Int J Pharm Bio Sci. 4, 1277?1283.
  8. Ajaz, S. A. Rasool, S. K. Sherwani and T. A. Ali. (2012). High profile chlorpyrifos degrading Pseudomonas putida MAS-1 from indigenous soil: Gas chromatographic analysis and molecular characterization. Int J Basic Med Sci Pharm. 2, 58?61.
  9. Vijayalakshmi and M. S. Usha. (2012). Isolation, screening and identification of bacteria capable of degrading chlorpyrifos and endosulfan. IJRPS, 2, 68?77.
  10. R. Naphade, A. A. Durve, M. Bhot, J. Varghese and N. Chandra. (2012). Isolation, characterization and identification of pesticide tolerating bacteria from garden soil. Eur J Exp Biol. 2, 1943?1951.
  11. B. Rokade and G. V. Malli. (2013). Biodegradation of chlorpyrifos by pseudomonas desmolyticum ncim 2112. Int J Pharm Bio Sci. 4, 609?616.
  12. Sharma, A. J. Pandit, R. Sharma and P. Shirkot. (2016). Biodegradation of chlorpyrifos by Pseudomonas resinovarans strain AST2.2 isolated from enriched cultures. Curr World Environ. 11, 267?278.
  13. Kavi Karunya and D. Reetha. (2012). Biological degradation of chlorpyrifos and monocrotophos by bacterial isolates. Int J Pharm Biolog Arch, 3, 685?691.
  14. R. El-Helow, M. E. I. Badawy, M. E. M. Mabrouk, E. A. H. Mohamed and Y. M. El-Beshlawy. (2013). biodegradation of chlorpyrifos by a newly isolated Bacillus subtilis strain, Y242. Bioremed J, 17, 113?123.
  15. S. Bakre, A. A. Sane, G. G. Biradar, and B. B. Kaliwal. (2008). Studies on isolation, characterization and growth of chlorpyrifos degrading bacteria from farm soil. South-Asian Journal of Multidisciplinary Studies. 3, 220-227.
  16. I. Eissa, H. A. Mahmoud, O. N. Massoud, K. M. Ghanem and I. M. Gomaa. (2014). Biodegradation of chlorpyrifos by microbial strains isolated from agricultural wastewater. J Am Sci, 10.
  17. Sharma, S. Saxena, A. Datta and S. Arora. (2016). Spectrophotometric analysis of degradation of chlorpyrifos pesticide by indigenous microorganisms isolated from affected soil. Int J Curr Microbiol App Sci. 5, 742?749.
  18. Gulati and M. M. Nisar. Isolation and characterization of chlorpyrifos utilizing bacteria from sugarcane field soil. J Pharm Biomed Sci. 5, 765?770.
  19. Sabdono, A. (2006). Biodegradation of chlorpyrifos by a marine bacterium Bacillus firmus Strain BY6 associated with branching coral Acropora sp. J Coastal Dev. 10, 1410?5217.
  20. Y. Liu, X. Chen, Y. Shi, and Z. C. Su. (2012). Bacterial degradation of chlorpyrifos by Bacillus cereus. J Adv Mater Res. 356, 676?680.
  21. Deng, Y. Chen, D. Wang, T. Shi, X. Wu, X. Ma, X. Li , R. Hua, X. Tang, and Q. X. Li. (2015). Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1. Journal of Hazardous Materials. 297, 17?24.
  22. C. Ifediegwu , K. C. Agu, N. S. Awah, A. E. Mbachu, C. B. Okeke, C. G. Anaukwu, P. O. Ubo, U. C. Ngenegbo, and C. M. Nwankwo. (2015). Isolation, growth and indentification of chlorpyrifos degrading bacterial from agriclutural soil in Anambra State, Nigeria. Universal Journal of Microbiology Research. 3, 46?52.
  23. Akbar and S. Sultan. (2016). Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement. Brazilian Journal of Microbiology. 47, 563?570.
  24. Yang, Y.H. Zhao, B. X. Zhang, C.H. Yang, and X. Zhang. (2005). Isolation and characterization of a chlorpyrifos and 3,5,6- trichloro-2-pyridinol degrading bacterium. FEMS Microbiol Lett. 251, 67?73.
  25. Jabeen, S. Iqbal1, and S. Anwar. (2015). Biodegradation of chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol by a novel rhizobial strain Mesorhizobium sp. HN3. Water and Environment Journal. 29, 151?160.
  26. Barathidasan, D. Reetha, D. J. Milton, N. Sriram and M. (2014). Govindammal. Biodegradation of chlorpyrifos by co-culture of Cellulomonas fimi and Phanerochaete chrysosporium. African Journal of Microbiology. 8, 961?966.
  27. Abraham, A. Shanker and S. Silambarasan. (2013). Role of Gordonia sp. JAAS1 in biodegradation of chlorpyrifos and its hydrolysing metabolite 3,5,6-trichloro-2- pyridinol. Let Appl Microbiol. 57, 510?516.
  28. A. Romeh and M. Y. Hendawi. (2014). Bioremediation of certain organophosphorus pesticides by two biofertilizers, Paenibacillus (Bacillus) polymyxa (Prazmowski) and Azospirillum lipoferum (Beijerinck). J Agr Sci Tech. 16, 265?276.
  29. Nagavardhanam and Z. Vishnuvardhan. (2012). Isolation, screening and identification of Organophosphate pesticide degrading Bacterium, Kocuria sp. Asian Journal of Biological and Life Sciences. 1, 204?207.
  30. Kumar S. (2011). Bioremediation of chlorpyrifos by bacteria isolated from the cultivated soils. International Journal of Pharma and Bio Sciences. 2, 359?66.
  31. Cappuccino and N. Sherman. (2010). Microbiology: A Laboratory Manual, 9 edn. San Francisco: Benjamin- Cummings Publishing Company, Subs of Addison Wesley Longman, Inc.
  32. G. Holt, N. R. Krieg, P. H. Sneath, J. T. Staley and S. T. Williams. (1994). Bergey?s Manual of Determinative Bacteriology.
  33. Neti and V. Zakkula. (2013). Analysis of chlorpyrifos degradation by Kocuria sp. using GC and FTIR. Current Biotica 6, 466?472.
  34. Vijayalakshmi and M.S. Usha. (2012). Optimization of chlorpyrifos degradation by Pseudomonas putida. J Chem Pharm Res, 4, 2532?2539.
  35. Hindumathy, Gayathri, V. (2013). Effect of pesticide (chlorpyrifos) on soil microbial flora and pesticide degradation by strains isolated from contaminated soil. Bioremediation and Biodegradation, 4, 178?1822.

[V. Hamsavathani, O. S. Aysha and A. Raughul Ajith. (2017); ISOLATION AND IDENTIFICATION OF CHLORPYRIFOS DEGRADING BACTERIA FROM AGRICULTURAL SOIL. Int. J. of Adv. Res. 5 (May). 1209-1221] (ISSN 2320-5407). www.journalijar.com

Hamsavathani
Assistant Professor, Department of Microbiology, Jaya College of Arts and Science, Thiruninravur and Research Scholar PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, Tamilnadu, India

DOI:

Article DOI: 10.21474/IJAR01/4234      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4234

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