ROLE OF SERUM NATURE ON IN VITRO GROWING OF PLASMODIUM FALCIPARU.M.
- Laboratoire de Pharmacodynamie-biochimique, UFR Biosciences, Universit? de Cocody, 22 BP 582 Abidjan 22.
- Laboratoire de biochimie, Institut Pasteur de C?te d?Ivoire, Bp V 490 Abidjan.
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Plasmodium falciparum, the liable parasite for the mortal malaria, is a major case of morbidness and of mortality in everybody tropical. The in vitro culture of the Plasmodium remains an indispensable method for determination of the proof phenotype and of the surveillance of the efficacity of antimalarial drugs. Culture of Plasmodium falciparum requires the use of RPMI 1640 medium, the efficacy of which depends on addition of human serum. Culture with medium containing either reference human serum (RS) or serum containing Plasmodium antibodies (SND) provides a plasmodial maturation rate exceeding 20% (lower threshold of validity according to WHO). Comparison of these two culture supplements for in vitro testing of the chemosensitivity of Plasmodium strains to pyrimethamine showed no difference in IC50: < 2000 nM for sensitive strains (FCB1 and PFB) and > 2000 nM for resistant strain (K1)
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[Kipr? Gueyraud Rolland, BAGRE Issa, Bla Kouakou Brice and Djaman Allico Joseph. (2017); ROLE OF SERUM NATURE ON IN VITRO GROWING OF PLASMODIUM FALCIPARU.M. Int. J. of Adv. Res. 5 (Jun). 200-203] (ISSN 2320-5407). www.journalijar.com
FELIX HOUPHOUET-BOIGNY UNIVERSITY
