15Jun 2017

EVALUATION OF ANTIBACTERIAL EFFECT OF AUTOLOGOUS PLATELET CONCENTRATES AGAINST PERIODONTAL DISEASE ASSOCIATED BACTERIA - AN INVITRO STUDY.

  • Department of Periodontics, College Of Dental Sciences, Davangere.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Aim: The aim of this study is to evaluate the antimicrobial effects of platelet‑rich plasma (PRP) and platelet‑rich fibrin (PRF) against periodontal disease associated bacteria at two different time intervals. Subjects and Methods: Blood samples were obtained from ten patients. Procurement of PRP and PRF were done using centrifugation. The antibacterial activity of PRP and PRF was evaluated by microbial culturing using bacterial strains of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis at 2nd and 7th days. Results: At 2nd and 7th day, both P. gingivalis and A. actinomycetemcomitans were inhibited by PRP but no zone of inhibition was seen by PRF. Conclusions: PRP can be used as a potential autologous concentrate against periodontal pathogens and also it may serve as an adjunct in the enhancement of periodontal regeneration.

  1. Saini R. A clinical and microbiological study to evaluate the effect of dietary supplement of coenzyme Q10 in nonsurgical treatment outcome of chronic periodontitis patients after phase 1 periodontal therapy. Eur J Gen Dent 2014;3:194‑
  2. El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, Alshahat M, van Dyke TE: Platelet-rich plasma: growth factors and pro- and anti-inflammatory properties. J Periodontol. 2007;78:661-669.
  3. Anitua E, Alonso R, Girbau C, Aguirre JJ, Murozabal F, Orive G: Antibacterial effect of plasma rich in growth factors (PRGF-Endoret) against Staphylococcus aureus and Staphylococcus epidermidis strains. Clin Exp Dermatol. in press.
  4. Badade PS, Mahale SA, Panjwani AA, Vaidya PD, Warang AD. Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin. Indian J Dent Res 2016;27:300-4.
  5. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T: Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leukocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009, 27:158?167.
  6. Gasling VLW, Acil Y, Springer IN, Hubert N, Wiltfag J (2009) Platelet-rich plasma and platelet-rich fibrin in human cell culture. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:45?48.
  7. Hirsch JG. Comparative bactericidal activities of blood serum and plasma serum. J Exp Med 1960;112:15?22.
  8. Hamzeh-Cognasse H, Damien P, Chabert A, Pozzetto B, Cognasse F, Garraud O. Platelets and infections ? Complex interactions with bacteria. Front Immunol 2015;6:82.
  9. Krijgsveld J, Zaat SA, Meeldijk J, van Veelen PA, Fang G, Poolman B, et al. Thrombocidins, microbicidal proteins from human blood platelets, are C‑terminal deletion products of CXC chemokines. J Biol Chem 2000;275:20374‑
  10. Yeaman MR. The role of platelets in antimicrobial host defense. Clin Infect Dis 1997;25:951‑
  11. Klinger MH, Jelkmann W. Role of blood platelets in infection and inflammation. J Interferon Cytokine Res 2002;22:913‑
  12. Tang YQ, Yeaman MR, Selsted ME. Antimicrobial peptides from human platelets. Infect Immun 2002;70:6524‑
  13. Tohidnezhad M, Varoga D, Podschun R, Wruck CJ, Seekamp A, Brandenburg LO, et al. Thrombocytes are effectors of the innate immune system releasing human beta defensin‑ Injury 2011;42:682‑6.
  14. Tohidnezhad M, Varoga D, Wruck CJ, Podschun R, Sachweh BH, Bornemann J, et al. Platelets display potent antimicrobial activity and release human beta‑defensin 2. Platelets 2012;23:217‑
  15. Moojen DJ, Everts PA, Schure RM, Overdevest EP, van Zundert A, Knape JT, Castelein RM, Cremers LB, Dhert WJ: Antimicrobial activity of plateletleukocyte gel against Staphylococcus aureus. J Orthop Res 2008, 26:404?410.
  16. Tohidnezhad M, Varoga D, Wruck CJ, Brandenburg LO, Seekamp A, Shakibaei M, S?nmez TT, Pufe T, Lippross S: Platelet-released growth factors can accelerate tenocyte proliferation and activate the antioxidant response element. Histochem Cell Biol 2011, 135:453?460.
  17. Trowbridge CC, Stammers AH, Woods E, Yen BR, Klayman M, Gilbert C. Use of platelet gel and its effects on infection in cardiac surgery. J Extra Corpor Technol 2005; 37:381?386.
  18. Englert SJ, Estep TH, Ellis-Stoll CC. Postoperative surgical chest and leg incision sites using platelet gel: A retrospective study. J Extra Corpor Technol 2008; 40:225?228.

[Bharath chandra gnr, Narayan n walavalkar, Anurag bhatnagar and Shobha prakash. (2017); EVALUATION OF ANTIBACTERIAL EFFECT OF AUTOLOGOUS PLATELET CONCENTRATES AGAINST PERIODONTAL DISEASE ASSOCIATED BACTERIA - AN INVITRO STUDY. Int. J. of Adv. Res. 5 (Jun). 462-466] (ISSN 2320-5407). www.journalijar.com

Bharath Chandra GNR

DOI:

Article DOI: 10.21474/IJAR01/4433      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4433

Download Full Paper

Download PDF No. of Downloads: 41 | No. of Views: 123