17Jul 2017

CARBOHYDRATE ASSIMILATION PROFILE OF TWO WILD STRAINS OF GENUS CANDIDA IN A MIXTURE OF HEXOSES AND PENTOSES BY ALCOHOL PRODUCTION.

  • Centro de Investigaci?n y Asistencia en Tecnolog?a y Dise?o del Estado de Jalisco, Tablaje Catastral 31264 Km 5.5 Carretera Sierra Papacal-Chuburna Puerto Parque Cient?fico Tecnol?gico de Yucat?n, Merida, Yucatan, M?xico. CP. 97302.
  • Centro de Biotecnolog?a Gen?mica del Instituto Polit?cnico Nacional, Boulevard del Maestro s/n esq. El?as Pi?a, Col. Narciso Mendoza, Cd. Reynosa, Tamaulipas, M?xico. C.P. 88710.
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Bioethanol is an alternative energy source used as fossil fuel complement. It can be obtained from microbial fermentation of sugars. Efficient fermentations require microorganisms capable of use all the carbohydrates present in raw materials but most of them have different preferences for its assimilation. Mixed cultures are an alternative to perform carbohydrate fermentations due the synergistic combination of different metabolic capacities. In this work the carbohydrate assimilation profile of two Candida glabrata strains (code T1 and LR2) isolated from termite gut and bovine ruminal fluid, respectively, was studied in single and mixed cultures on sugar mixtures. A minimal medium based on nitrogen with 120 g/l of carbohydrates (in proportions similar to those found in citric residues) was inoculated with 2.0 x 107 cell/ml. In mixed cultures (code MC1 and MC2), strains were inoculated at proportion 50:50 with the same inoculum concentration. Fermentations were made during ten days at 35?C (by T1 single culture and MC1) and 40?C (by LR2 single culture and MC2) at 200 rpm. LR2 had higher cellular population and biomass and T1 had better growth rate and doubling time. All the cultures assimilate all sugars. MC1 had the best assimilation of glucose (94.81?0.12%), fructose (83.83?0.60%) and arabinose (62.12?0.42%). T1 single culture had the best assimilation of galactose (52.64?0.71%) and xylose (56.75?0.66%). T1 and MC1 had the best ethanol production (24.28 ?0.78 g/l and 24.32 ?1.00 g/l, respectively). MC2 had the highest maximum productivity (6.98 ? 0.73 g/l?day) and alcohol production rate (0.128?0.004 g/lh).

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[Eduardo Jos? Burgos-Valencia, Jos? Alberto Narv?ez-Zapata, Guadalupe L?pez-Puc, Manuel Octavio Ram?rez-Sucre and Ingrid Mayanin Rodr?guez-Buenfil. (2017); CARBOHYDRATE ASSIMILATION PROFILE OF TWO WILD STRAINS OF GENUS CANDIDA IN A MIXTURE OF HEXOSES AND PENTOSES BY ALCOHOL PRODUCTION. Int. J. of Adv. Res. 5 (Jul). 192-204] (ISSN 2320-5407). www.journalijar.com

Ingrid Mayanin Rodríguez-Buenfil1
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Tablaje Catastral 31264 Km 5.5 Carretera Sierra Papacal-Chuburna Puerto Parque Científico Tecnológico de Yucatán, Merida, Yucatan, México. CP. 97302.

DOI:

Article DOI: 10.21474/IJAR01/4708      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4708

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