28Jul 2017

ANTI-ULCER ACTIVITY OF TERMINALIA LAXIFLORA ENGL. AND DIELS LEAVES EXTRACTS AGAINST ASPIRIN INDUCED GASTRIC ULCER IN RATS.

  • Department of Biology, Center of Basic Sciences, Misr University for Science and Technology, 6th October City.
  • Departments of Phytochemistry & Plant Systematic.
  • Chemistry of Natural & Microbial Products.
  • Pathology, National Research Centre, Dokki, Cairo, Egypt.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Background/Aim: Gastric ulcer is one of the most widespread gastrointestinal disorders resulting from weakness of a gastric mucosal defense. Several drugs are available to address ulcer; however, these drugs are associated with several side effects. Previous studies have confirmed the efficacy of herbal plants extracts for possible treatment of this disease. This research aims to evaluate the antiulcer properties of Terminalia laxiflora extracts. Methods: Water and its fractions, total methanol, dichloromethane, ethyl acetate, butanol extracts from the leaves of Terminalia laxiflora were evaluated for their antiulcer activity using aspirin as ulcerogen in rats. All administration was given one hour prior to aspirin. Macroscopic, hisopathological and histochemical examinations were performed the effect of these extracts on ulcer. Results: All extracts showed significant inhibitory activity. The percentages of inhibition for the different extracts equal to 54.21, 64.86, 61.71, 76.84, 75.92, 79.47, and 86.05 % for water 1, water 2, methanol, DCM, ethyl acetate, and butanol as compared to non-pretreated aspirin group. Butanol extract was found to possess remarkable ulcer-protective properties at 50 mg/kg when compare to other the other extracts. The effect of ulcer protection 86.05 % was produced and standard drug, Ranitidine, gave 81.57% of ulcer protection, respectively. Conclusion: We conclude that Terminalia laxiflora extracts are potential sources of new antiulcer agents.

  1. Abdallah IZA, Khattaba AHK, Heebab GH. (2011). Gastroprotective effect of Cordiamyxa fruit extract against indomethacin-induced gastric ulceration in rats. Life Science Journal 8: 433-445.
  2. Abdulla MA, Al-Bayaty FH, Younis LT, Abu Hassan MI. (2010), Antiulcer activity of Centella asiatica leaf extract against ethanol induced gastric mucosa injury in rats. J Med Plant Res 4 (13): 1253-1259.
  3. Akuodor GC, Mbah CC, Essien AD, Akpan JL, Ezeokpo BC, Iwuanyanwu TC, et al. (2012): Ulcer-protective and antidiarrhoeal effects of the aqueous stem bark extract of Bridelia ferruginea in rodents. Pharmacolgia 3 (11): 591-597.
  4. Alkofahi, A. and AH. Atta, (1999): Pharmacological screening of the antiulcerogenic effects of some Jordanian Mecicinal Plants in rats. J. Ethnopharmacol, 65: 341-345.
  5. Ariypshi, I., Toshiharu, A., Sugimura, F., Abe, M., Matsuo, Y., and Honda, T. (1986) Recurrence during maintenance therapy with histamine H2 receptors antagonist in cases of gastric ulcers. Nikon University Journal of Medicine, 28: 69-74
  6. Ashley S, Sonnenschein L, Cheung L. (1985) :Focal gastric mucosal blood flow at the site of aspirin-induced ulceration. Am J Surg 149:53-59.
  7. Carlo GD, Mascolo N, lzzo AA, Capasso F. (1999):Flavonoids: Old and new aspects of a class of natural therapeutic drugs.?Life Sci.? 65:337?353.
  8. De Barros, M.P., Lemos, M., Maistro, E.L., Leite, M.F., Sousa, J.P.B., Bastos, J.K., de Andrade, S.F. (2008): Evaluation of antiulcer activity of the main phenolic acids found in Brazilian Green Propolis. Journal of Ethnopharmacology, 120(3), 372-377.‏
  9. De La Lastra, C.A., Martin, M.J., Motilva, V. (1994): Antiulcer and gastroprotective effects of quercetin: a gross and histologic study. Pharmacology, 48(1), 56-62.‏
  10. Dela Pureta R., Martinez-Dominguez E., Ruiz-Gutierrez V., (2000): Effect of minor components of virgin olive oil on topical anti-inflammatory assays, Zetschrift Fur Naturforschong 55 814?819.
  11. Dubey, S., Ganeshpurkar, A., Shrivastava, A., Bansal, D., Dubey, N. (2013): Rutin exerts antiulcer effect by inhibiting the gastric proton pump. Indian journal of pharmacology, 45(4), 415.‏
  12. Farzaei M.H., Abdollahi, M., Rahimi R. (2015): Role of dietary polyphenols in the management of peptic ulcer. World J Gastroenterol.??21(21): 6499?6517
  13. Freitas CS, Baggio CH, Finau J, Anginoni M, Pizzolatti MG, Santos ARS, Marquez MCA. (2008): Inhibition of H+/K+ ATPase in the gastroprotective effect of Baccharis illinita DC. J Pharm Pharmacol. 60:1105-10.
  14. Govindarajan R, Vijayakumar M, Singh M, Rao ChV, Shirwaikar A, Rawat AK, et al. (2006): Antiulcer and antimicrobial activity of?Anogeissus latifolia.?J Ethnopharmacol.? 106: 57?61.
  15. Gupta, ,Mazumder, U.K.,?Manikandan L.,?Bhattacharya S.,?Senthilkumar G.P.,? Suresh R. (2005): Anti-ulcer activity of ethanol extract of?Terminalia pallida?Brandis. in Swiss albino rats. Journal of Ethnopharmacology; 97(2): 405?408
  16. Gupta, R.K., Al-Shafi, S., Layden, K. and Haslam, E. (1982): The metabolism of gallic acid and hexahydroxydiphenic acid in plants. Part 2. Esters of (S)-hexahydroxydiphenic acid with D-glucopyranose (4C1)'', J. Chem. Soc. Perkin Trans I, 2525.
  17. Haddock, A., Gupta, R.K., Al-Shafi, S. and Haslam, E. (1982): The metabolism of gallic acid and hexahydroxydiphenic acid in plants. Part 1. Introduction. Naturally occurring galloyl esters. Chem. Soc. Perkin Trans I, 2515.
  18. Harborne, J. B. and William, C. A. (1975): ''The Flavonoids'' Chapman and Hall, London
  19. Izzo, A.A., Carlo, G.D., Mascolo, N., Capasso, F. (1994): Antiulcer effect of flavonoids. Role of endogenous PAF. Phytotherapy Research, 8(3), 179-181.
  20. Kandaswami C, Middleton E. (1994): Free radical scavenging and antioxidant activity of plant flavonoids.?Adv Exp Med Biol.? 366:351?376.
  21. Kitahora T, Guth PH. (1987): Effect of aspirin plus hydrochloric acid on the gastric mucosal microcirculation. Gastroenterology 93:810-817.
  22. Kumar A, Dewan B, Rama T. (2011): Evaluation of anti-ulcerogenic properties from the root of Flemingia strobilifera. J Basic Clin Pharm. 2(1).
  23. Laine L, Takeuchi K, Tarnawski A. (2008): Gastric mucosal defense and cytoprotection: bench to bedside. Gastroenterology 135:41?60.
  24. Mard S.A, Mojadami S., Farbood Y., Kazem M., Naseri G. (2015):?The anti-inflammatory and anti-apoptotic effects of gallic acid against mucosal inflammation- and erosions induced by gastric ischemia-reperfusion in rats. Vet Res Forum.? 6(4): 305?311.
  25. Martin M. J., Motilva,V. Alarc, de la Lastra C. (1993):Quercetin and naringenin; effects on ulcer formation and gastric secretion in rats. Phytotherapy Research, 7(2): 150?153.
  26. Jawanjal H,Rajput MS,?Agrawal P,?Dange V. (2012): Pharmacological evaluation of fruits of Terminalia belerica Roxb. for antiulcer activity. J Complement Integr Med.? 9 (1): 1?
  27. Mohiuddin, A.K.M.; Islam, Maidul; Mahmud, Shahin; Apu, Md. Aminul Islam, Halder, Joyanta, Khoka, Md. Sadek Hosen; Rakib, Hasibul Haque; Shome, Binita; Islam, Md. Shariful, A. (2015): comprehensive biological, ethnopharmacological and phytochemical update review on ayurvedic plant of Terminalia? chebula (HORTOKI) of Bangladesh, World Journal of Pharmaceutical Research 4(5), 386-405.
  28. Mota KSdL, Dias GEN, Pinto MEF, Luiz-Ferreira A, Souza-Brito ARM, Hiru?ma-Lima CA, Barbosa-Filho JM, Batista LM. (2009):Flavonoids with Gastroprotective Activity. Molecules. 14:979-1012.
  29. Muddathir A M, Yamauchi K, Mitsunaga T, (2013): Anti-acne activity of tannin-related compounds isolated from Terminalia laxiflora, J Wood Sci 59:426?431.
  30. Muscara MN, Wallace JL. Nitric oxide. V. (1999): Therapeutic potential of nitric oxide donors and inhibitors. Am J Physiol 276:G1313?G1316.
  31. Nassar MI, Mohamed TK, Elshamy AI, El-Toumy SA, Abdel Lateef AM, Farrag AH. (2013):Chemical constituents and anti-ulcerogenic potential of the scales of Cynara scolymus (artichoke) heads. J Sci Food Agric, 93: 2494?2501.
  32. Olaleye SB, Farombi EO. (2006): Attenuation of indomethacin and HCI/ethanol-induced oxitative gastric mucosa damage in rats by Kolaviron: A natural biflavonoid of Garcina kola seed. Phytothera Res 20: 14.
  33. Pillai, N.R., Suganthan, D., Seshari, C., and Santhakumari, G. (1978): Antigastric ulcer activity of nimbidin. Indian Journal of Medical Research, 68: 169-175.
  34. Rashed K, Ono L.(2013): Phytochemical, Cytotoxic, anti- HSV-1 (Herpes Simplex Virus type- 1) and anti bacterial studies of Terminalia laxiflora and Diels, Hygeia. J. D. Med. vol.5 (2): 76-86.
  35. Robins PG. (1980): Ultrastructural observations on the pathogenesis of aspirin induced gastric erosions. Br J Exp Path01 61: 497-504.
  36. Rotelli AE, Guardia T, Ju?rez AO, de la Rocha NE, Pelzer LE. (2003): Comparative study of flavonoids in experimental models of inflammation.?Pharmacol Res.? 48:601?6.
  37. Rowe PH, Starlinger MJ, Kasdon E, Hollands MJ, Silen W. (1987): Parenteral aspirin and sodium salicylate are equally injurious to the rat gastric mucosa. Gastroenterology 93:863-871.
  38. Scheiman JM, Dubois, Giardiello FM. (1996): NSAIDs, Eicosonoids and the Gastroenteric Tract. Sounders: Philadelphia, 25: 102-108.
  39. Sen, S., Asokkumar, K., Umamaheswari, M., Sivashanmugam, A., Subhadradevi, V. (2013): Antiulcerogenic effect of gallic acid in rats and its effect on oxidant and antioxidant parameters in stomach tissue. Indian journal of pharmaceutical sciences, 75(2), 149.‏
  40. Sen,?S. Asokkumar,?K. Umamaheswari,? M. Sivashanmugam,?T. and?Subhadradevi V. (2013): Antiulcerogenic Effect of Gallic Acid in Rats and its Effect on Oxidant and Antioxidant Parameters in Stomach Tissue. Indian J Pharm Sci. 75(2): 149?155.
  41. Shirode D, Patel T, Pal Roy S, Jyothi TM, Rajendra SV, Prabhu K, Setty SR. (2008):Anti-ulcer properties of 70% ethanolic extract of leaves of Albizzia lebbeck. Phcog Mag. 4:228-31.
  42. Suh, W.S.; Kim, Y.S. Schimmer, A.D.; Kitada, S. Minden, M.; Andreeff, M., Suh, N.; Sporn, M. Reed, J.C. (2003): Synthetic triterpenoids activate a pathway for apoptosis in AML cells involving downregulation of FLIP and sensitization to TRAIL, Leukemia 17: 2122?2129.
  43. Sumbul, S., Ahmad, M. A., Asif, M., & Akhtar, M. (2011): Role of phenolic compounds in peptic ulcer: An overview. Journal of Pharmacy and Bioallied Sciences, 3(3), 361.‏
  44. Suzuki Y., Hayashi M., Ito M., and Yamagami I. (1976): Anti ulcer effects of 4? (2 carboxyethyl) phenyl trans 4 aminomethyl cyclohexanecarboxylate hydrochloride (cetraxate) on various experimental gastric ulcers in rats, Japanese Journal of Pharmacology, 26(4), 471?480.
  45. Suzuki, Y., Ishihara, M., Segami, T., & Ito, M. (1998): Anti-ulcer Effects of Antioxidants, Quercetin,. ALPHA.-Tocopherol, Nifedipine and Tetracycline in Rats. The Japanese Journal of Pharmacology, 78(4), 435-441.‏
  46. Szabo S. (1987): Mechanisms of mucosal injury in the stomach and duodenum: time-sequence analysis of morphologic, functional, biochemical and histochemical studies. Stand J Gastroenterol 22(127):21-28.
  47. Tepperman BL, Jacobson ED. (1994):Circulatory factors in gastric mucosal defense and repair. In Physiology of the Gastrointestinal Tract, Johnson LR (ed.). Raven Press: New York.
  48. Toma, W., Hiruma-Lima, C.A. Guerrerand, R.O. and Souza A.R. (2005): Preliminary studies of Mammea mericana L (Guttiferae) bark/latex extract point to an effective antiulcer effect on gastric ulcer models in mice, Phytomedicine, 12: 345-350.
  49. Wallace JL, Miller MJ. (2000): Nitric oxide in mucosal defense: a little goes a long way. Gastroenterology 119: 512?520.
  50. Wang M, Lee E, Song W, Ricciotti E, Rader DJ, Lawson JA, Pur? E, FitzGerald GA. (2008): Microsomal prostaglandin E synthase-1 deletion suppresses oxidative stress and angiotensin II-induced abdominal aortic aneurysm formation. Circulation 117: 1302?1309.
  51. Yuan Y, Padol IT, Hunt RH. (2006): Peptic ulcer today. Nat Clin Pract Gastr. 3:80?89.
  52. Zilic S, Serpen A, Akıllıog lu G, Gökmen V, Vancetovic J. (2012):Phenolic Compounds, Carotenoids, Anthocyanins, and Antioxidant Capacity of Colored Maize (Zea mays L.) Kernels. J Agric Food Chem, 60:1224−1231.

[Medhat M. Menshawy, Mohamed A. El Raey, Abdel Hamid A. Hamdy and Abdel Razik H. Farrag. (2017); ANTI-ULCER ACTIVITY OF TERMINALIA LAXIFLORA ENGL. AND DIELS LEAVES EXTRACTS AGAINST ASPIRIN INDUCED GASTRIC ULCER IN RATS. Int. J. of Adv. Res. 5 (Jul). 2092-2100] (ISSN 2320-5407). www.journalijar.com

Medhat Mansour Menshawy Shoala
Misr University For Science And Technology

DOI:

Article DOI: 10.21474/IJAR01/4946      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4946

Download Full Paper

Download PDF No. of Downloads: 22 | No. of Views: 91