17Jul 2017

STUDY ON THE PRODUCTION AND ANTIBACTERIAL ACTIVITIES OF BIOSURFACTANT PRODUCED FROM SOME BACTERIAL SPECIES.

  • Environmental Biology Dept, Federal Polytechnic NekedeOwerri, Imo-State.
  • Biotechnology Dept, Federal University of Technology Owerri, Imo-State.
  • Chemistry Dept, Federal University of Technology Owerri, Imo-State.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Biosurfactant are produced by some micro organisms. Kerosene was used as substrate to enhance the production of biosurfactant by Staphylococcus aureus, Bacillus sp., Pseudomonas aeruginosa, Proteus sp. and Corynebacteriumsp. Staphylococcus aureus produced the highest 0.5 g, Bacillus sp., Pseudomonas aeruginosa and Proteus sp. 0.2 g, Corynebacterium sp. the least 0.1 g. The biosurfactant demonstrated antibacterial activity against the test bacteria (Staphylococcus sp. and Pseudomonas aeruginosa). The biosurfactant produced by Pseudomonas aeruginosa gave the highest zone of inhibition against Staphylococcus aureus, Bacillus sp., Corynebacteriumsp., (25 mm). The biosurfactant produced by Staphylococcus aureus gave the highest zone of inhibition against Pseudomonas aeruginosa (39 mm), Corynebacteriumsp., and Proteus sp. (30 mm), Bacillus sp. the least zone (25 mm). The production of biosurfactantand antibacterial efficacy can thus be promising for use in medical, therapeutics, pharmaceuticals, cosmetcs, food and beverages for treatment and control of diseases caused by micro organisms.

  1. Abalos, A., Pinazo, A., Infante, M. R., Casals, M., Garcıa, F., and Manresa, A., (2001). Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes. Langmuir. 17(5):1367-1371.
  2. Ahimou, F., Jacques, P., and Deleu, M., (2001). Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enzyme and Microbial Technology. 1(27):749-754.
  3. Anandaraj, B., and Thivakaran, P., (2010). Isolation and production of biosurfactant producing organisms from oil spilled soil. Biosci Tech. 1(3):120-126.
  4. Calvo, C., Manzanera, M., Silva-Castro, G.A., Uad,I., and Gonzalez-Lopez, J., (2009). Application of biomemulsifiers in soil oil bioremediation processes. Future prospects. Sci. Total Environ. 407:3634-3640.
  5. Carrilo, C., Teruel, J.A., Aranda, F.J. and Ortiz, A. (2003) . Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochimica et Biophysica Acta. 1611: 91-97.
  6. Chakrabarti, S., (2015). Bacterial biosurfactant: Characterization, antimicrobial and metal remediation properties. Dissertation submitted in partial fulfillment of the requirement for the degree of Master of Science in life science. Department of life science national institute of technology Rourkela Odisha. pp. 1-62
  7. Christofi, N., and Ivshina, I., (2002). Microbial surfactants and their use. In soil remediation book: Bioremediation of aquatic and terrestrial ecosystems. pp. 311-327.
  8. Dhail, S., and Jasuja, N., (2012). Isolation of biosurfactant-producing marine bacteria. African Journal of Environmental Science and Technology. 6(6):263-266. DOI: 10.5897/AJEST12.068.
  9. Fernandes, A., Arruda, I.R., Santos, A.F.A.B., Ara?jo, A., Maior, A.M., and Ximenes, E.A., (2007). Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug resistant bacteria. Brazilian Journal of Microbiology. 38:704-709.
  10. Franzetti, A., Gandolfi, I., Bestetti, G., Smyth, T.J., and Banat, I.M., (2010). Production and applications of trehalose lipid biosurfactants. J. Lipid. Sci. Tech. 112:617?627.
  11. Govindammal, M., and Parthasarathi, R., (2013). Investigation on antimicrobial activity of biosurfactant produced by Pseudomonas fluorescens isolated from the mangrove ecosystem. International Research Journal of Pharmacy. 4(1):230-232 http://www.iriponline.com
  12. Gudi?a, E.J., Rocha, V., Teixeira, J.A., and Rodrigues, L.R., (2010). Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei Paracasei A20. Applied Microbiology. 50:419-424.
  13. Hassanshahian, M., (2014). Isolation and characterization of biosurfactant producing bacteria from Persian Gulf (Bushehr provenance). Marine Pollution Bulletin 86:361?366.
  14. Jinfeng, L., Lijun, M., Bozhong, M., Rulin, L., Fangtian, N., and Jiaxi, Z., (2005). The field pilot of microbial enhanced oil recovery in a high temperature petroleum reservoir. Petrol. Sci. Eng.48:265?271.
  15. Joseph, P.J., and Joseph, A., (2009). Microbial enhanced separation of oil from a petroleum refinery sludge. Hazard. Mater. 161:522?525.
  16. Joshi, S., Bharucha, C., and Desai, A.J., (2008b). Production of biosurfactant and antifungal compound by fermented food isolate subtilis 20B. Biores. Technol. 99:4603-4608.
  17. Khare, E., and Arora, N., (2011). Physiochemical and structural characterization of biosurfactant from fluorescent Pseudomonas with biocontrol activity against Macrophomina phaseolina In: Proceedings of the 2nd Asian PGPR conference August 21-24 2011, Beijing P.R. China.
  18. Liu, T., Hou, J., Zuo, Y., Bi, S., and Jing J., (2011). Isolation and characterization of a biosurfactant producing bacterium from Daqing oil-contaminated sites. African Journal of Microbiology Research. 5(21):3509-3514. DOI: 10.5897/AJMR11.684.
  19. Muthusamy, K., Gopalakrishnan, S., Ravi, T.K., and Sivachidambaram, P., (2008). Biosurfactants: Properties, commercial production and application. Current Science. 94:736-774.
  20. Okore, C. C., Nwaehiri, L. U., Mbanefo, O.N., Ogbulie, T. E., Ugenyi, A. U., Ogbuka, I. B., Ejele A. E. and Okwujiako I. A. (2017a). Study on effect of monovalent and divalent salts on the production of biosurfactant and emulsification index. I J. Adv. Res. 5(6) : 1875-1881, Doi:10.21474/IJAR01/4616, ISSN: 2320-5407
  21. Okore, C. C., Nwaehiri, L. U., Mbanefo, O.N., Ogbulie, T. E., Ugenyi, A. U., Ogbuka, I. B., Ejele A. E. and Okwujiako I. A. (2017b). Study on microbial diversity of biosurfactant producing bacteria from contaminated enviromental samples. J. Adv. Res. 5(6): 1387-1396, Doi:10.21474/IJAR01/4548, ISSN: 2320-5407.
  22. Okore, C.C., Mbanefo, O.N., Onyekwere, B.C., Onyewenjo, S., Abba-Father, C.A.M. (2013). Isolation and Characterization of Biosurfactants Producing Bacteria from Oil Polluted Soil?,Journal of Natural Sciences Research iiste.org. ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online). 3(5) pp 119-122
  23. Osadebe, P.O. and Ukwueze, S.E. (2004). A comparative study of the phytochemical and antimicrobial properties of the eastern Nigerian species of African mistletoe (Loranthus micranthus) sourced from different host trees. Journal of Biological Research and Biotechnology, 2:18 -23
  24. Płociniczak, M.P., Płaza, G.A., Seget, Z.P., and Cameotra, S.S., (2011). Environmental Applications of Biosurfactants: Recent Advances. J. Mol. Sci., 12:633-654. Doi 10.3390/ijms 12010633.
  25. Priya, T., and Usharani, G., (2009). Comparative study for biosurfactant production by using Bacillus subtilis and Pseudomonas aeruginosa. Botany Research International. 2(4):284-287 ISSN 2221-3635.
  26. Rodrigues, L.R, Moldes, A, Teixeira JA, Oliveira R. (2006a). Kinetic study of fermentative biosurfactant production by Lactobacillus Biochemical Engineering Journal. 28:109-116.
  27. Rodrigues, L.R, Teixeira, J.A, Van Der Mei H.C., Oliveira R., (2006b). Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus Colloids and Surfaces B: Biointerfaces. 53:105 -112.
  28. Rodrigues, L.R., Banat, I.M., Van der Mei, H.C., Teixeira, J.A., and Oliveira, R., (2006c). Interference in adhesion of bacteria and yeasts isolated from explanted voice prostheses to silicone rubber by rhamnolipidbiosurfactants. Journal of Applied Microbiology. 100(3):470-480.
  29. Saisa-Ard, K., Maneerat, S., and Saimmai, A., (2013). Isolation and characterization of? biosurfactants-producing bacteria isolated from palm oil industry and evaluation for biosurfactants production using low-cost substrates. BioTechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology.? 94(3):275-284.
  30. Santa Anna, M., Sebastian, G.V., Menezes, E.P., Alves, T.L.M., Santos, A.S, Pereira Jr., N., and Freire, D.M.G., (2002). Production of biosurfactants from Pseudomonas aeruginosa PA1 isolated in oil environments. Brazilian Journal of Chemical Engineering. 19:(2): 159-166.
  31. Santa Anna, I.M., Sebastian, G.V., Pereira, J.R.N., Alves, T.L.M., Menezes, E.P., and Freire, D.M.G., (2001). Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa Appl. Biochem. Biotechnol. 91:459?67. http://dx.doi.org/10.1385/ABAB:91-93:1-9:459.
  32. Sifour, M., Al-Jilawi, M.H., and Aziz, G.M., (2007). Emulsification properties of biosurfactant produced from Pseudomonas aeruginosa Pak. J. Biol. Sci. 10:1331?1335.
  33. Tambekar, D.H., and Gadakh, P.V., (2013). Biochemical and molecular detection of biosurfactant producing bacteria from soil. J. Life Sc. Biotechnology and Pharma. Research. 2(1):204-211.
  34. Tolls, J., Haller, M., Seinen, W., Sijm, D.T., and LAS. H.M., (2000). Bioconcentration: Tissue distribution and effect of hardness implications for processes. Sci. Technol. 34:304-10.
  35. Venhuis, S. H., and Mehrva, M., (2004). Health effects, environmental impacts, and photochemical degradation of selected surfactants in water. International Journal of Photoenergy. 2:115-125.

[Chioma Okore, Linda Nwaehiri, Ogechukwu Mbanefo, Toochukwu Ogbulie, Assumpta Ugenyi, Ifeanyi Ogbuka, Agunna Ejele and Ikechukwu Okwujiako. (2017); STUDY ON THE PRODUCTION AND ANTIBACTERIAL ACTIVITIES OF BIOSURFACTANT PRODUCED FROM SOME BACTERIAL SPECIES. Int. J. of Adv. Res. 5 (Jul). 581-586] (ISSN 2320-5407). www.journalijar.com

CHIOMA OKORE
Environmental Biology Dept, Federal Polytechnic Nekede Owerri, Imo-State.

DOI:

Article DOI: 10.21474/IJAR01/4755      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4755

Download Full Paper

Download PDF No. of Downloads: 19 | No. of Views: 88