20Jul 2017

SCREENING OF ROOT NODULE BACTERIA FROM WILD LEGUMES FOR THEIR POSSIBLE ROLE IN PLANT GROWTH PROMOTION.

  • Department of Biotechnology,Career college, Bhopal, India.
  • Department of Biochemistry,Career college, Bhopal, India.
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In the present study, root nodule bacteria (RNB) were studied for their plant growth promoting (PGP) properties. For recovery of RNB wild leguminous plants viz., Abrus precatorious (A. precatorious), Crotolaria retusa (C. retusa), Crotolaria turnesia (C. turnesia), Desmodium sp. Gliricidia sp and Mimosa pudica (M. pudica) were collected from different areas of Bhopal. A total of 31 isolates (7 from A. precatorious, 3 from C. retusa, 4 from C. turnesia, 15 from Desmodium sp., 1 from Gliricidia sp. and 1 from M. pudica) were recovered and were studied for PGP activities viz., siderophore production, phosphate solubilization (P.solubilization), Indole acetic acid (IAA) production and ACC deaminase activity. 45.1% of the recovered isolates were positive for siderophore production with isolate DM1e showing maximum production i.e. 72.6 ?g ml-1. About 22.5% of the isolates produced IAA with isolate DM1f showing the maximum production of 10 ?g ml-1. P. solubilization and ACC deaminase activity was recorded in 54.8 and 87% isolates respectively. Further study on nitrogen fixation is underway. RNB such as rhizobia are known to promote plant growth by fixing nitrogen within root nodules and provide it to the plant for nutrition. Multifaceted beneficial effects can be obtained on plant growth and health by such RNB if they possess additional PGP properties as worked out in this study. Moreover, benefits can be obtained even if they fail to nodulate the plant (a constraint for rhizobia) but colonize the rhizosphere and compete with indigenous microbial population. Such microbes with multiple PGP properties could be developed as bioinoculants and have the potential to fetch the profit in growing biotechnology sector of India.

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[Nidhi Gujar, Shaishavee Saini, Preeti Chandurkar, Tanuja Murab, Anjali Choudhary and Nidhi Tripathi. (2017); SCREENING OF ROOT NODULE BACTERIA FROM WILD LEGUMES FOR THEIR POSSIBLE ROLE IN PLANT GROWTH PROMOTION. Int. J. of Adv. Res. 5 (Jul). 992-998] (ISSN 2320-5407). www.journalijar.com

Nidhi Gujar
Assistant Professor

DOI:

Article DOI: 10.21474/IJAR01/4808      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4808

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