18Sep 2017

AMELIORATIVE ROLE OF BONE MARROW MESENCHYMAL STEM CELLS AND RUTIN AGAINST ACETAMINOPHEN INDUCED HEPATOTOXICITY IN ADULT MALE RATS.

  • Zoology Department, Women?s College for Arts, Science and Education ? Ain Shams University, Egypt.
  • Molecular Biology and Tissue Culture, Faculty of Medicine, Medical Research Center, Ain Shams University Cairo, Egypt.
  • Zoology Department, Faculty of Science, Omar AL-Moukhtar University, EL-Beida, Libya.
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The present study was undertaken to evaluated the therapeutic effect of bone marrow mesenchymal stem cells (MSCs) and/or rutin against acetaminophen induced hepatotoxicity in adult male albino-rats. Rats were divided into 5 groups: Group 1 (-ve control group) with no treatment; Group 2 (Acetaminophen recovery group)rats were given orally acetaminophen 750 mg/kg b.wt every 72h for two weeks then left for 60 days without any treatment ; Group 3 (Acet +rutin group) rats were given orally acetaminophen 750 mg/kg b.wt every 72h for two weeks then treated orally with rutin 25 mg/kg b.wt daily for 8 weeks ; Group 4 (Acet + MSCs) acetaminophen group then injected intravenously with a single dose of MSCs (1X10 6 ) cell / cm2 saline /animale ) and Group 5 (Acet +rutin and MSCs treated group) rats were given (750 ) mg/kg b.wt Acettaminophen every 72h for two weeks then injected intravenously with a single dose of MSCs (1X10 6 ) cell / cm2 saline /animale ) and rutin. Acetaminophen adminstration caused significant increase in serum liver enzymes (AST,ALT, ALP & γ GT) , inflammatory Cytokinase ( TNFα , IL-6 & IL-1β ) and lipid peroxidation MDA with decrease in liver Cytochrom P450 antioxidant enzymes and oxidative stress (liver SOD and GSH) as compared to control group. On the other hand , administration of rutin and /MSCs exhibited marked amelioration in the previous parameters as compared to control group. The present study revealed that intravenous transplantation of bone marrow mesenchymal stem cells (BMSCs) or rutin administration are both successful methods for the improvement of liver functions in rats after the overdose effects of acetaminophen ?induced oxidative stress and inflammatory injury via retaining hepatic GSH,SOD activity ,MAD content and decreasing production of IL-6,IL-1β ,TNF-alph, cytochrom P450 and liver enzymes .

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[Walaa A. M. El-Nahrawy, Fatma A. Abu Zahra and Rabha E. A. EL-Dab (2017); AMELIORATIVE ROLE OF BONE MARROW MESENCHYMAL STEM CELLS AND RUTIN AGAINST ACETAMINOPHEN INDUCED HEPATOTOXICITY IN ADULT MALE RATS. Int. J. of Adv. Res. 5 (Sep). 450-459] (ISSN 2320-5407). www.journalijar.com

Walaa A.M. El-Nahrawy
Zoology Department, Women’s College for Arts, Science and Education – Ain Shams University, Egypt.

DOI:

Article DOI: 10.21474/IJAR01/5349      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5349

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