28Jul 2017

SPECIES IDENTIFICATION INFERRED THROUGH MITOCHONDRIAL 16S rRNA GENE SEQUENCE BETWEEN FRESHWATER TESTUDINES - LISSEMYS PUNCTATA AND MELANOCHELYS TRIJUGA.

  • Molecular Endocrinology Laboratory, Department of Biotechnology, Yuvaraja?s College, A Constituent Autonomous College of University of Mysore, Mysore ? 570005.
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The molecular study of mitochondrial 16S rRNA gene is the first comparative report showing molecular variations of the target gene between two freshwater terrapins- Lissemys punctata and Melanochelys trijuga. Partial 16S rRNA gene amplification was performed by hotstart PCR and sequences were acquired by bidirectional Sanger sequencing after column purification of amplicons. Interspecies comparison after sequence alignment of database species from two families along with query sequences revealed 54% (total 918 sites) conserved regions between the families and only 44% showed variations. Intraspecies conserved/variable regions were 1359/84 and 1404/42 sites out of 1696 total positions considered respectively. The presence of consensus regions among different species indicates the scope for developing universal primers to amplify the region in various species of turtles belonging to these families. Our results highlight that 16S rRNA sequences were successful in identifying and evaluating the species into their respective family Trionychidae and Geoemydidae. Hence, it is recommended to conduct similar study on these species in large numbers concentrating various geographical locations of Karnataka, India to curb wildlife crimes and conserve these species in the ecosystem.

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[Lalitha R and Vidya R Chandavar. (2017); SPECIES IDENTIFICATION INFERRED THROUGH MITOCHONDRIAL 16S rRNA GENE SEQUENCE BETWEEN FRESHWATER TESTUDINES - LISSEMYS PUNCTATA AND MELANOCHELYS TRIJUGA. Int. J. of Adv. Res. 5 (Jul). 2231-2239] (ISSN 2320-5407). www.journalijar.com

Vidya Chandavar R
Co-Author

DOI:

Article DOI: 10.21474/IJAR01/4964      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4964

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