20Jul 2017

PROTECTIVE/ POTENTATIVE ACTION OF CAFFEINE IN CHROMIUM TREATED SWISS MICE MALE GERM LINE CELLS.

  • Lecturer in Zoology, U.P. Science College, Sheragada, Ganjam, Odisha.
  • Lecturer in Zoology, Nuapada College, Ganjam, Odisha.
  • Retd. Professor, Department of Zoology, Berhampur University Berhampur-760007.
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Chromic acid, a hexavalent compound of chromium is known to induce reproductive dysfunctions in male laboratory animals and humans. Reduced semen quality, formation of morphologically abnormal sperm population and significant reduction in sperm count are the chief reproductive deformities resulted due to significantly higher oxidative stress accompanied with marked decrease in antioxidant enzymes in the testes of Cr-exposed individuals. In the present study, an attempt has been taken to investigate the Protective/Potentiate effect of caffeine, a plant product, in reducing Chromium induced testicular cytotoxicity in chromium treated Swiss mice. In this contest, three sub-lethal doses of CAF 25, 50 & 100 mg/kg body weight were tested in mouse followed by Chromic acid injection. Results indicate that CAF pretreatment in 25 mg/kg potentially reduced oxidative stress in terms of lipid peroxidation (LPP), marked increase in sperm count and significant decline in abnormal sperm population. Contrarily, Cr- treated mice pretreated with 100 mg of CAF reflected in higher LPP compared to controls. Also, population of aberrant sperm was significantly higher with a significant decrease in sperm count compared to controls. Furthermore, the efficacy of CAF is also compared with other antioxidant vitamins like Vit-C &Vit-E. It is also inferred that lower dose of CAF is comparable to VIT-E rather that Vit-C in ameliorating the Cr-induced testicular dysfunction in mice.

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[Manoj Kumar Panda, Rabi Narayan Pradhan and U. R. Acharya. (2017); PROTECTIVE/ POTENTATIVE ACTION OF CAFFEINE IN CHROMIUM TREATED SWISS MICE MALE GERM LINE CELLS. Int. J. of Adv. Res. 5 (Jul). 1235-1241] (ISSN 2320-5407). www.journalijar.com

Prof. Usha rani Acharya
Retd. Professor, Department of Zoology, Berhampur University Berhampur-760007

DOI:

Article DOI: 10.21474/IJAR01/4832      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4832

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