24Jul 2017

ISOLATION AND IDENTIFICATION OF BIOSURFACTANT PRODUCING CHROMATE RESISTANT BACTERIA ISOLATED FROM CHROMIUM CONTAMINATED SITE.

  • Department of Microbiology Dr RML Avadh University, Faizabad, 224 001, India.
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The occurrence of chromate tolerant and biosurfactant producing bacteria with an ability to bioremediate Cr(VI) through bioreduction was investigated in the soil of waste dumping site of battery manufacturing industry. Two bacterial strains TB9 and TB10 showed high Cr(VI) MIC values of 1350 and 1400 ?g/ml, respectively, and also reduced 83% and 90.0% of chromate after 20 h of growth. Interestingly, both the strains were capable to produce biosurfactant in significant amount which was confirmed by methylene blue plate assay, blood hemolysis test, emulsification test and oil displacement activity. The TB9 strain showed highest emulsification index (E24) after 24 hours of incubation for sunflower oil (74?1.60) followed by kerosene (72?0.5) and olive oil (65?0.3), whereas, strain TB10 showed highest E24 value for olive oil (74?1.2) followed by sunflower oil (70?0.29) and kerosene (68?0.19) after the same period of exposure. Results for oil spreading test of the kerosene oil revealed that TB9 and TB10 caused the spreading of the oil at the rate of 6 and 5 minutes with a diameter of 2.7 cm and 3.3 cm, respectively. Based on the results of concomitant biosurfactant production efficiency and chromate reduction potential, strains TB9 and TB10 seems to be most efficient strains that can be potentially be used for Cr(VI) bioremediation and were identified as Pseudomonas aeruginosa and Bacillus cereus, respectively.


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[Tuhina Verma. (2017); ISOLATION AND IDENTIFICATION OF BIOSURFACTANT PRODUCING CHROMATE RESISTANT BACTERIA ISOLATED FROM CHROMIUM CONTAMINATED SITE. Int. J. of Adv. Res. 5 (Jul). 1515-1522] (ISSN 2320-5407). www.journalijar.com


Tuhina Verma
Department of Microbiology Dr RML Avadh University, Faizabad, 224 001, India

DOI:


Article DOI: 10.21474/IJAR01/4865      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4865