Influence of beta-glucan on Genotoxic effects of silver nanoparticles on immunized mice

Abstract: In order to investigate the influence of beta-glucan and augment immune response on genotoxicity of Silver-nanoparticles,40 white mice ,both sexes average age 8-10 weeks were randomly divided into 4 groups equally and treatment as following: 1st group was administrated with 20m\\kg BW of Silvernanoparticles(Ag-NPs) 20nm in size, for 6 weeks,2nd group was immunized with o.3ml of whole sonicated Salmonella hader(protein concentration 2mg\\ml .S\\C two dose ,two weeks interval and at same time administrated with Ag-NPs as in 1st group,3rd group was administrated with Ag-NPs as in 1st group and at same time was immunized as in 2nd group and fed diet supplementing with 2g\\kg diet of beta glucan,4th group was administrated orally with o,3ml of sterile saline for 6 weeks and served as control negative group. At the end of the experiment ,all the animals were sacrificed and bone marrow samples were taken for demonstration of blastic,mitotic index .micronuclei and chromosomal aberration. The result showed low ratio of blastic and mitotic index with high number of chromosomal aberration and micronuclei in animals treated with Ag-NPs ,and the ratio of blastic index and mitotic index in cells of bone marrow cells of immunized animals –Ag-NPs were higher than those value in 1st group but less in 3rd group, few to moderate or absent of chromosomal aberration and micronuclei were recorded in immunized animals with or without fed diet supplementing with beta –glucan and administrated Ag-NPS. It was concluded that Ag-NPs have genotoxic effects and augment immune response with Beta-glucan could diminished these toxic effects.

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[Jihad A.J & Alwan M.J. (2014); Influence of beta-glucan on Genotoxic effects of silver nanoparticles on immunized mice Int. J. of Adv. Res. 2 (6). 0] (ISSN 2320-5407). www.journalijar.com

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