C677T and A1298C polymorphisms of Methylenetetrahydrofolate Reductase Gene in Iraqi Patients with Recurrent Abortion

Abstract: Recurrent spontaneous abortion (RSA) was significant obstetrical complications that may occur during pregnancy. Various studies in recent years have indicated that two common mutations (C677T and A1298C) of the methylenetetrahydrofolate reductase (MTHFR) gene were risk factor for RSA. This study was carried out to determine the influence of (C677T and A1298C) of the methylenetetrahydrofolate reductase (MTHFR) gene mutations in Iraqi women with RSA. A total of 80 women were included in this study: fifty women with two or more consecutive miscarriages and 30 healthy controls. Total genomic DNA was isolated from blood leukocytes and the frequency of the two common C677T and A1298C MTHFR gene mutations in the patients and controls was determined used PCR-restriction fragment length polymorphism (PCR-RFLEP).There was no significant difference in the prevalence of 677T/T genotype among women with RSA and healthy controls (P = 0.37). Also no statistically significant difference in the frequency of A1298C MTHFR gene mutation was detected between the two groups (P=0.23). In conclusion, the results indicated no significant difference in MTHFR C677T/A1298C genotype distribution among the two groups; therefore, further studies on larger population and other genetic variants to better understand the pathobiology of RSA were needed.

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[Hasan F.Al-Azzawie and Sara Sabri (2014); C677T and A1298C polymorphisms of Methylenetetrahydrofolate Reductase Gene in Iraqi Patients with Recurrent Abortion Int. J. of Adv. Res. 2 (6). 0] (ISSN 2320-5407). www.journalijar.com

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