30Sep 2017

ANTIMICROBIAL POTENTIALS OF SPORE CULTURE OF GEASTRUM SP., A RARE WILD EDIBLE MUSHROOM OF SIMILIPAL BIOSPHERE RESERVE, ODISHA, INDIA AGAINST SOME SIGNIFICANT HUMAN PATHOGENS.

  • Department of Botany and Biotechnology, North Orissa University, Baripada, Odisha, India.
  • Department of Biotechnology, North Orissa University, Baripada, Odisha, India.
  • Mycology and Plant pathology Laboratory Dept. of Botany, Gauhati University, Assam-781014.
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A rarely found Geastrum species was collected from Shorea robusta forest soil of Similipal Biosphere Reserve, Odisha, which is known for its ethnomedicinal uses as wound healing. Fungal mycelia developed from spore culture of the fruiting body were used to evaluate antimicrobial activities. The spore mass of the macro fungi were aseptically inoculated to Potato Dextrose Agar medium to obtain the mycelia. Three different fungal media (viz. PDA, MEA, CDA) were used to study the radial growth. Further the fungal mycelium was cultivated in three liquid media (viz. PDB, MEB, CDB) and antimicrobial activity of the cultural broth was evaluated at different incubation periods (viz. 7 days, 14 days, 21 days) against six bacterial and three fungal clinically significant human pathogens. The crude metabolites of the fungus cultivated in PDB medium was extracted using ethyl acetate as solvent after 14days of incubation for further antimicrobial and phytochemical analysis.Maximum radial growth of the fungus was observed in Potato dextrose medium and maximum antimicrobial activity was observed in PDB medium as compared to other two media after 14 days of incubation against both bacterial and fungal human pathogens. Qualitative screening of the extract showed presence of phytochemicals. The crude metabolites showed ? max of 1.76 indicating presence of active compounds. The present study demonstrates successful development of mycelia from the macro-fungal spore of Geastrum sp., and reports considerable antimicrobial activity of the fungal metabolites which needs to be isolated and characterized.


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[Mrunmaya K Panda, Hrudayanath Thatoi and Kumananda Tayung. (2017); ANTIMICROBIAL POTENTIALS OF SPORE CULTURE OF GEASTRUM SP., A RARE WILD EDIBLE MUSHROOM OF SIMILIPAL BIOSPHERE RESERVE, ODISHA, INDIA AGAINST SOME SIGNIFICANT HUMAN PATHOGENS. Int. J. of Adv. Res. 5 (Sep). 1508-1516] (ISSN 2320-5407). www.journalijar.com


Kumananda Tayung
Associate Professor at Dept. of Botany, Gauhati University, Assam-781014

DOI:


Article DOI: 10.21474/IJAR01/5473      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5473