COMPARISION OF EFFECT ON THE POTENCY OF BACITRACIN SECRETED BY BACILLUS PUMILUS, BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS MUTATED BY ULTRAVIOLET RADIATION AND HEAVY METALS

Present study is carried out to show a comparison of the potency check of Bacitracin produced by Bacillus pumilus, Bacillus subtilis and Bacillus licheniformis treated with the Ultra Violet radiation and heavy metals. These microbes were isolated from the Sewage Treatment plant of MUST residential campus, Lakshmangarh, Sikar. Molecular identification of isolates was done by DNA isolation, PCR amplification using 16s rDNA primer and partial sequencing of purified product after sequencing. Sequences were submitted in NCBI with designated accession no. There is growing awareness of the need for development of new antimicrobial agents for the treatment of human, animal and plant diseases. A special fermentation media, given by Hanlon & Hodges, was used for the production of Bacitracin. Micrococcus luteus was used as a test organism. Zones of inhibition were observed of both wild and mutant strains. A standard Zinc Bacitracin (70 IU/ml) was taken for calculating potency of the sample Bacitracin. The potency of antibiotic Bacitracin (IU/ml) was determined by agar diffusion method given by William. Results indicated that the potency was highest of B.subtilis and least by B.licheniformis. This study is made to use native or indigenous micro flora for bioremediation which is useful and beneficial rather than commercial inoculum. Bacillus itself will be enough for the biodegradation of the complex molecules.