01Jan 2017

Emergence of Chloramphenicol-florfenicol resistance (cfr) gene mediated linezolid resistance in methicillin resistant Staphylococcus aureus, case reports and review of literature.

Abstract


Context: Despite the use of linezolid, resistance to it has remained stable and extremely low with only sporadic cases have been reported. Aim: To find out linezolid resistance in MRSA isolates. Material and Methods: Specimens were collected aseptically and cultured using standard microbiological protocols. Antibiotic susceptibility was done by Kirby-Bauer disc diffusion method according to CLSI guidelines. Minimum inhibitory concentration (MIC) of these isolates were carried out and confirmed by E-test. PCR was performed for mec A, nuc A and cfr genes. Sequencing of the linezolid resistant isolates was also carried out. Results: We here report three cases of cfr mediated linezolid resistant MRSA isolated from patients. MIC of linezolid to each Staphylococcus aureus was ?8 µg/ml. The MIC of linezolid was further confirmed by E –test. PCR was done for amplification of mec A, nuc A and cfr genes confirming MRSA and cfr mediated linezolid resistance. Conclusion: Scarcity of newer antimicrobials and increasing resistance to available drugs demands implementation of infection control measures to prevent the dissemination of cfr gene mediated linezolid resistant Staphylococcus aureus.


Cite This Article as:


[Bashir Ahmad Fomda, Asiya Khan, Danish Zahoor, Javid Rasool, Haroon Rashid Zargar (2014); Emergence of Chloramphenicol-florfenicol resistance (cfr) gene mediated linezolid resistance in methicillin resistant Staphylococcus aureus, case reports and review of literature. Int. J. of Adv. Res. 2 (10). 0] (ISSN 2320-5407). www.journalijar.com


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