10Oct 2017

TWO STEP TRANSFER OF RYE-DERIVED HESSIAN FLY- RESISTANCE GENE H21 TO DURUM WHEAT BY COMPENSATING ROBERTSONIAN TRANSLOCATION AND INDUCED HOMOEOLOGOUS RECOMBINATION.

  • Moha Ferrahi, National Institute for Agricultural Research, Regional Center of Meknes, BP 578, Meknes, Morocco 50000.
  • B. Friebe and B.S. Gill, Dept. Of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
  • J.H. Hatchett, Dept. of Entomology and USDA-ARS, Waters Hall, Kansas State University, Manhattan, KS 66506-5502, USA.
  • G.L. Brown-Guedira, Dept. Of Agronomy and USDA-ARS, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The Hessian fly resistance gene H21 is present on the wheat-rye whole-arm translocation T2BS?2RL and was recently transferred to durum wheat. However, homozygous lines for this translocation have poor plant vigor, low seed set, and are almost completely sterile, making it impossible to use this germplasm directly in durum wheat improvement. The objective of this study was to reduce the rye segment in T2BS?2RL using ph-mediated recombination, thereby making this gene available for durum breeding. A total of 39 primary recombinants from a population of 512 plants (7.6%) were recovered involving 10% of the distal segment of the long arm of rye or wheat. Among these primary recombinants 21 (53.8%) had distal rye chromatin, 12 (30.8%) had distal wheat chromatin, the remaining four had either very distal wheat or rye and two were interstitial recombinants. Ten out of 39 primary recombinants were tested for their resistance to Hessian fly. Three recombinants (Rec. # 1, 2, 3) with about the distal 10% of 2BL arm derived from rye reacted resistant and, thus, had the H21 gene. Two recombinants (Rec. # 4, 5) with very distal rye chromatin reacted susceptible to the Hessian fly. Three other primary recombinants (Rec. # 6, 7, 8) with the distal 10% of the 2RL arm derived from wheat were susceptible to Hessian fly and did not retain the H21 gene. The remaining two recombinants (Rec. # 9, 10) with very distal wheat segments were resistant and still had the resistance gene H21. The distal primary recombinants with the gene were vigorous and had normal seed set and can now be used in the improvement of durum wheat.

  1. Aragon-Alcaide, L., S. Reader, A. Beven, P. Shaw, T. Miller, and G. Moore. 1997. Association of homologous chromosomes during floral development. Curr Biol 7: 905-908.
  2. Ceoloni, C., M. Biagetti, M. Ciaffi, P. Forte, and M. Pasquiri. 1996. Wheat chromosome engineering at the 4x level: the potential of different alien gene transfers into durum wheat. Euphytica 89: 87-97.
  3. Devos, K.M., M.D. Atkinson, C.N. Chinoy, H.A. Francis, R.L. Harcourt, R.M.D. Koebner, C.J. Liu, P. Masojc, D.X. Xie, and M.D. Gale. 1993. Chromosome rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85: 673-689.
  4. Dvorak, J., and A.J. Lukaszewski. 2000. Centromere association is an unlikely mechanism by which the wheat Ph1 locus regulates metaphase I chromosome pairing between homoeologous chromosomes. Chromosoma 109: 410-414.
  5. Dvorak, J., M.M. Noaman, S. Goyal, and J. Gorham. 1994. Enhancement of the salt tolerance of Triticum turgidum by the Kna1 locus transferred from Triticum aestivum L. chromosome 4D by homoeologous recombination. Theor Appl Genet 87: 872-877
  6. Dvorak, J., and J. Gorham. 1992. Methodology of gene transfer by homoeologous recombination into Triticum turgidum: transfer of K+/Na+ discrimination from Triticum aestivum. Genome 35: 639-646.
  7. El Bouhssini, M., O. Benlahbib, M.M. Nachit, A. Houari, A. Bentika, N. Nsarellah, and S. Lhaloui. 1998. Identification in Aegilops species of resistant sources to Hessian fly (Diptera: Cecidomyiidae) in Morocco. Genetic Resources and Crop Evolution 45: 343-345.
  8. Feldman, M. 1993. Cytogenetic activity and mode of action of the pairing homoeologous (Ph1) gene of wheat. Crop Sci 33: 894-897.
  9. Friebe, B., J.H. Hatchett, R.G. Sears, and B.S. Gill. 1990. Transfer of Hessian fly resistance from ?Chaupon? rye to hexaploid wheat via a T2BS?2RL wheat-rye chromosome translocation. Theor Appl Genet 79: 385-389.
  10. Friebe, B., J.H. Hatchett, B.S. Gill, Y. Mukai, and E.E. Sebesta. 1991. Transfer of Hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations. Theor Appl Genet 83: 33-40.
  11. Friebe, B., M. Heun, N. Tuleen, F.J. Zeller, and B.S. Gill. 1994. Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci. 34: 621-625.
  12. Friebe, B., J. Jiang, W.J. Raupp, R.A. McIntosh, and B.S. Gill. 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91: 59-87.
  13. Friebe, B., R.G. Kynast, J.H. Hatchett, R.G. Sears, D.L. Wilson, and B.S. Gill. 1999. Transfer of wheat-rye translocation chromosomes conferring resistance to Hessian fly from bread wheat into durum wheat. Crop Sci 39: 1692-1696.
  14. Gill, B.S., B. Friebe, and T.R. Endo. 1991. Standard Karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34: 830-839.
  15. Gill, K.S., and B.S. Gill. 1994. Mapping in the realm of polyploidy: The wheat model. BioEssays vol. 16, No.11: 841-846.
  16. Giorgi, B. 1978. A homoeologous pairing mutant isolated in Triticum durum Cappelli. Mutat. Breed. Newsletter. 11: 4-5.
  17. Hatchett, J.H., and R.L. Gallun. 1970. Genetics of the ability of the Hessian fly, Mayetiola destructor, to survive on wheats having different genes for resistance. Ann Entomol Soc Am 63: 1400-1407.
  18. Hatchett, J.H., T.J. Martin, and R.W. Livers. 1981. Expression and inheritance of resistance to Hessian fly in synthetic hexaploid wheats derived from Triticum tauschii (Coss) Schmal. Crop Sci 21: 731-734.
  19. Jauhar, P.P., O. Riera-Lizarazu, W.G. Dewey, B.S. Gill, C.F. Crane, and J.H. Bennett. 1991. Chromosome pairing relationships among the A, B, and D genomes of bread wheat. Theor Appl Genet 82: 441-449.
  20. Jauhar, P.P., A.B. Almouslem, T.S. Peterson, and L.R. Joppa. 1999. Inter- and intragenomic chromosome pairing in haploids of durum wheat. Hered. 90(4): 437-445.
  21. Jiang, J., B. Friebe, and B.S. Gill. 1994a. Chromosome painting of Amigo wheat. Theor Appl Genet 89: 811-813.
  22. Jiang, J., B. Friebe, and B.S. Gill. 1994b. Recent advances in alien gene transfer in wheat. Euphytica 73: 199-212.
  23. Joppa, L.R. 1993. Chromosome engineering in tetraploid wheat. Crop Sci 33(5): 908-913.
  24. Kimber, G., and R. Riley. 1963. The relationships of the diploid progenitors of hexaploid wheat. Can J Genet Cytol 5: 83-88.
  25. Lhaloui, S., M. El Bouhssini, M.M. Nachit, N. Nsarellah, and A. Amri. 1998. New sources of resistance to Hessian fly in Wheat in Morocco. Vol. 3: 287-289. In A.E. Slinkard (ed.) Proc Intl Wheat Genet Symp, 9th. Saskatoon, SK. University Extension Press, University of Saskatchewan, Saskatoon.
  26. Lukaszewski, A.J. 1993. Reconstruction of complete chromosomes 1B and 1R from the 1RS?1BL translocation of ?Kavkaz? origin. Genome 36: 821-824.
  27. Lukaszewski, A.J. 1995. Physical distribution of translocation breakpoints in homoeologous recombinants induced by the absence of the Ph1 gene in wheat and triticale. Theor Appl Genet 90: 714-719.
  28. Lukaszewski, A.J. 1997. Further manipulation by centric misdivision of the 1RS?1BL translocation in wheat. Euphytica 94: 257-261.
  29. Lukaszewski, A.J. 2000. Manipulation of the 1RS?1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40: 216-225.
  30. Luo, M-C, Dubcovsky, and J. Dvorak. 1996a. Recognition of homoeology by the Ph1 Genetics 143: 1195-1203.
  31. Luo, M-C, Dubcovsky, S. Goyal, and J. Dvorak. 1996b. Engineering of interstitial foreign chromosome segments containing the K+/Na+ selectivity gene Kna1 by sequential homoeologous recombination in durum wheat. Theor Appl Genet 93: 1180-1184.
  32. Martinez M., T. Naranjo, C. Cuadrado, and C. Romero. The synaptic behavior of Triticum turgidum with variable doses of the Ph1 locus. Theor Appl Genet 102: 751-758.
  33. Martines-Perez E., P. Shaw, S. Reader, L. Aragon-Alcaide, T. Miller, and G. Moore. 1999. Homologous chromosome pairing in wheat. J Cell Sci 112: 1761-1769.
  34. McIntosh, R.A., G.E. Hart, K.M. Devos, M.D. Gale, and W.J. Rogers. 1998. Catalogue of gene symbols for wheat. Vol. 5: 1-236. In A.E. Slinkard (ed.) Proc Intl Wheat Genet Symp, 9th. Saskatoon, SK. University Extension Press, University of Saskatchewan, Saskatoon.
  35. Mikhailova, E.I., T. Naranjo, K. Shephered, J. Wennekes-van Eden, C. Heyting, and H. de Jong. 1998. The effect of wheat Ph1 locus on chromatin organization and meiotic pairing analyzed by genome pairing. Chromosoma 107: 339-350.
  36. Naranjo, T., P. Fernandez-Rueda. Pairing and recombination between individual chromosomes of wheat and rye in hybrids carrying the ph1b mutation. Theor Appl Genet 93: 242-248.
  37. Nsarellah, N., M.M. Nachit, M. El Bouhssini, S. Lhaloui, and A. Amri. 1998. Introgression of Hessian fly resistance into durum wheat in Morocco. Vol. 3: 303-304. In A.E. Slinkard (ed.) Proc Intl Wheat Genet Symp, 9th. Saskatoon, SK. University Extension Press, University of Saskatchewan, Saskatoon.
  38. Okamoto, M. 1957. Asynaptic effect of chromosome V. Wheat Info Service 5:6.
  39. Ratcliffe, R.H., and J.H. Hatchett. 1997. Biology and genetics of the Hessian fly and resistance in wheat. New developments in Entomology, pp: 47-55.
  40. Riley, R., and V. Chapman. 1958. Genetic control of the cytologically diploid behavior of hexaploid wheat. Nature 182: 713-715.
  41. Riley, R., V. Chapman, and R. Johnson. 1968a. The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis. Genet Res 12: 199-219.
  42. Riley, R., V. Chapman, and R. Johnson. 1968b. Introduction of yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217: 383-384.
  43. Roberts, M.A., S.M. Reader, C. Dalgliesh, T.E. Miller, T.N. Foote, L.J. Fish, J.W. Snape, and G. Moore. 1999. Induction and characterization of Ph1 wheat mutants. Genetics 153: 1909-1918.
  44. Sears, E.R. 1976. Genetic control of chromosome pairing in wheat. Ann Rev Genet 10: 31-51.
  45. Sears, E.R. 1977. An induced mutant with homoeologous pairing in common wheat. Can J Genet Cytol 19: 585-593.
  46. Sears, E.R. 1993. Use of radiation to transfer alien segments to wheat. Crop Sci 32: 897-901.

[Moha Ferrahi, B. Friebe, J. H. Hatchett, G. L. Brown-Guedira and B. S. Gill. (2017); TWO STEP TRANSFER OF RYE-DERIVED HESSIAN FLY- RESISTANCE GENE H21 TO DURUM WHEAT BY COMPENSATING ROBERTSONIAN TRANSLOCATION AND INDUCED HOMOEOLOGOUS RECOMBINATION. Int. J. of Adv. Res. 5 (Oct). 262-270] (ISSN 2320-5407). www.journalijar.com

Moha Ferrahi
National Institute for Agricultural Research, Regional Center of Meknes, BP 578, Meknes, Morocco 50000.

DOI:

Article DOI: 10.21474/IJAR01/5529      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5529

Download Full Paper

Download PDF No. of Downloads: 14 | No. of Views: 84