30Jun 2014

Identification of microsatellite loci in Channa marulius (Channidae: Perciformes) through cross species amplification

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C. marulius, which belongs to the family Channidae, has high commercial value and medicinal importance, categorized under lower risk- near threatened status (LRnt). The present investigation identified seven polymorphic microsatellite loci namely, CA05, CHA5, CHA13, CHM91, CHM123, CHM151 and CHM 176 and twenty monomorphic loci from three different cross species of Channa. The observations showed that the submitted microsatellite sequence of C. argus, C. maculate and C. striata could be used to identify microsatellite loci in C. marulius. Only seven primers which amplified bands at the expected allele size regions were identified as polymorphic microsatellite loci.


[Atul Bhalla, S.N. Shukla, R.M. Mishra and U.K. Chauhan (2014); Identification of microsatellite loci in Channa marulius (Channidae: Perciformes) through cross species amplification Int. J. of Adv. Res. 2 (6). 0] (ISSN 2320-5407). www.journalijar.com


ATUL BHALLA


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