30Jun 2014

In vitro propagation method of Ficus carica at Taif governorate using tissue culture technique

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The experiment has been conducted to achieve a method for multiple-shoot induction with apical buds collected from mature tree of Ficus carica L., family Moraceae using Murashige and Skoog’s (MS) medium supplemented with different growth regulators giberellic acid (GA3), benzyladenine (BA), kinetin (Kin). Data indicated that (BA) at the rate of 5 mg/L and (GA3) at 1 mg/L enhanced shoot multiplication. The best number of leaves was obtained from medium supplemented with 3 mg/L (BA) combined with (GA3) at 1 mg/L. In addition, plant length increased when (GA3) was added alone at 1 mg/L, followed by (GA3) combined with either (Kin) at 5 mg/L or (BA) at 3 mg/L compared with the control and the difference was significant.


[Hadeer Y. Darwesh, Saleh A. Bazaid and Basem N. Abu Samra (2014); In vitro propagation method of Ficus carica at Taif governorate using tissue culture technique Int. J. of Adv. Res. 2 (Jun). 0] (ISSN 2320-5407). www.journalijar.com


Dr. Hadeer Y. Darwesh