Antioxadative and antigenotoxic effects against cytotoxicity of thiamethoxam on mice

In order to determine the effect of thiamethoxam on cytotoxicity on mice,20 white female mice 9-10 weeks aged were divided randomly into two groups equally and treated as the following: the 1st group was administrated orally with 83.7 mg\\kg BW of thiamethoxam daily for 8 weeks, 2nd group was administrated orally with 0.4 ml of normal saline daily for 8 weeks and served as control negative group. All animals were sacrified at 8 weeks post-treatment, blood samples were taken for hormonal and biochemical examination, in addition bone marrow samples were taken for cytogenetic examination. The result showed that the ratio of mitotic index,blastic index and micronuclei higher (3.50 ± 0.15, 38.90 ± 1.21 and3.27± 0.31) respectively than those values in control negative group ( 2.18±0.6 , 24.30 ± 0.2,0.55±0.15) respectively. The current result expressed high levels of serum peroxynitrite and malondialdhyde radical concentration (M\\L) on thiamethoxam treated group at 4 and 8 weeks post-treatment (2.40 ± 0.006, 3.49 ± 0.002;2.50 ± 0.007, 3.59 ± 0.0073 respectively) were higher than those values (0.24 ± 0.005, 0.25 ± 0.0036; 0.30 ± 0.0051,. 0.35 ± 0.0036 respectively ) in control negative group ..In addition the result showed that thiamethoxam lead to significant decrease values of Estradal (E2) followed by ,Follicular stimulating hormone (FSH) ,luteinizing hormone(LH), and progesterone (20.20 ± 0.25, 9.05± 0.089, 3.66 ± 0.027, 1.25 ± 0.077respectively ).as compared with control group (25.54 ± 0.18. 12.83 ± 0.47, 6.10 ± 0.036, 2.80± 0.025respectively ), at 8weeks post-treatment with thiamethoxam. It was concluded that the thiamethoxam had oxidative, genotoxic and hormonal effects.