Potency Evaluation of Pseudomonas strains against root-knot nematode infecting Tomato

The rhizobacteria strains such as Pseudomonas with known ability to induce systemic resistance against different soilborne pathogens were studied in split-root experiments for their ability to induce systemic resistance against the root-knot nematode Meloidogyne incognita in tomato. This study attempted to construct more efficient soil bacterial strains may be able to controlling nematodes, as well as, maintenance the soil fertility using different strains of Pseudomonas to improve the biological control against the nematodes number in infected fields, in order to reduce the use of nematocidial agents. Four Pseudomonas strains were used as biocontrol agents against Meloidogyne incognita greenhouse. Data of plant growth parameters such as fresh and shoot weight and nematode reproduction in term of egg masses per root system, galls per root system, one gm of root and females per root system were recorded. The plants treated with P. putida8 appeared significantly increased in suppressed females per root. The results revealed appeared significant increase in total protein content at 60 days plant-old above uninoculated plants and plants infected by nematodes among the season. On the other hand, the results appeared higher accumulation of phenols plants tissue treated with Pseudomonas strains in response to invasion by root-knot nematodes collectively contribute to induced systemic resistance and decrease in nematode infection. The highest phenols accumulation was observed in plants treated byP.putida8 and PF- 23932 respectively.