Selection of Highly Efficient (siRNA) Targeting Membrane Genes in Helicobacter pylori 26695

Helicobacter pylori a liable influence risk of carcinogenesis that is a reason of most of mortality worldwide which has developed resistance to drugs. The present study was carried out to identify potential drug targets in H. pylori by design of potent siRNA. To reach the goal, the collection membrane essential gene for H. pylori from DEG, then a variety of bioinformatics databases studying STRING v9.1 for selection non-homologous genes, then the software to siRNA Sfold and MEGA6 to determine similarity between siRNA as groups. Further studies were carried out to list out essential protein of the H. pylori, approximately six (6) membrane proteins become drug target, than synthesized siRNA which are shorter than 21 nt about 9027 siRNA, 66 siRNA (following three rules: Ui-Tei, Amarzguioui and Reynolds), 29 siRNA groups 17 of them have many similar siRNA other are singe siRNA (MEGA6), 9 siRNA(cleavage sites), 5 siRNA Drug target (cleavage sites stability rule), this steps respectively. Conclusion: five (5) siRNA which could use as potential drug target for resistant of Helicobacter pylori 26695.