01Dec 2018

COMPARISON BETWEEN EFFECT OF LONG AGONIST AND ANTAGONIST PROTOCOLS ON MICRO-RNA- 320 LEVEL IN FOLLICULAR FLUID DURING IVF- ICSI.

  • M.B.ch.B/ MSc. Applied Embryology. High Institute for Infertility Diagnosis & Assisted Reproductive Technology / Al - Nahrain University.
  • M.B.ch. B/M.S.c, Ph.D Molecular Pathology,Medical collage/ Al- Nahrain University.
  • High Institute for Infertility Diagnosis & Assisted Reproductive Technology.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Background:The natural cycle (unstimulated cycle) was used in first IVF trial which give birth of a life baby. However, it was soon recognized that the success rate of IVF in natural cycles was low, this may be due to the low number of oocytes retrieved. Ovarian stimulation using urinary gonadotropins was applied to deal with this problem, resulting in a significant increase in both the number of eggs retrieved, as well as the success rate of IVF. But in the same time the using of stimulation drugs may negatively affect oocyte and embryo quality which could diagnosed by using non invasive procedure like measurement of miRNA- 320 in follicular fluid. Aim of study:To compare the effect of stimulation protocol on ICSI outcome using miRNA- 320 that reflects the embryo quality. Materials and method:This comparative study was done on the embryos of seventy one infertile females who were undergoing Intracytoplasmic sperm injection (ICSI) treatment regardless to the presence or absence of previous trials. These patients were divided into two groups, 45 of them treated with agonist protocol and other 26 patients treated with antagonist protocol. Of the 71 oocytes, there were eight immature oocytes (3 at germinal vesicle (GV) stage, 5 at metaphase I (MI) stage) and three abnormal oocytes, and these were excluded from the following ICSI treatment. Measurement of follicular fluid mi-RNA 320 (which is known biomarker of embryo quality) was done by PCR. Results:The statistical analysis showed no significant correlation between miRNA- 320 levels in follicular fluid and age, BMI, number of dominant follicles, number of oocytes retrieved, number of metaphase I, metaphase II, germinal vesicle, abnormal oocytes and number of fertilized oocytes (2PN). The comparison between miRNA 320 levels in good and bad quality embryos showed highly significant (p<0.001) difference but no significant difference (p>0.05) was found between the different stimulation protocols. Conclusions:The stimulation protocol does not affect the level of miRNA which is molecular marker of embryo quality.


  1. Blockeel C., Devroey P. Optimisation of the follicular phase in IVF/ICSI. FVV in ObGyn. 2012; 4 (3): 203-212
  2. Catt J. Effect of Ovarian Stimulation Protocols on Oocyte and Embryo Quality. In: Ghumman S. (eds) Principles and Practice of Controlled Ovarian Stimulation in ART. New Delhi: Springer, 2015.chapter Pp.558-569
  3. Cupisti S, Dittrich R, Mueller A,? Strick R,? Stiegler E,? Binder H, Beckmann M. W., StrisseP.Correlations between anti-mullerian hormone, inhibin B, and activin A in follicular fluid in IVF/ICSI patients for assessing the maturation and developmental potential of oocytes. Eur J Med Res. 2007; 12, 604.
  4. da Silveira JC, Veeramachaneni DN, Winger QA, Carnevale EM, Bouma GJ. Cell-secreted vesicles in equine ovarian follicular fluid contain miRNAs and proteins: a possible new form of cell communication within the ovarian follicle. BiolReprod. 2012; 86:71.
  5. Diez-Fraile A, Lammens T,?Tilleman K,?Witkowski W,?Verhasselt B,?De Sutter P,?Benoit Y,?Espeel M,?D\'Herde K. Age-associated differential microRNA levels in human follicular fluid reveal pathways potentially determining fertility and success of in vitro fertilization. Hum Fertil (Camb) . 2014; 17, 90.
  6. Feng R, Sang K, Zhu Y, Fu W, Liu M, Xu Y, Shi H, Xu Y, Qu R, Chai R, Shao R, JinL, He L, Sun X& Wang L.. MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro. Scientific report. 2015; 5: 8689. P1-9
  7. Kim V.N. MicroRNA biogenesis: Coordinated cropping and dicing. Rev. Mol. Cell Biol. 2005; 6: 376.
  8. L?d?e N,?Lombroso R,?Lombardelli L,?Selva J,?Dubanchet S,?Chaouat G,?Frankenne F,?Foidart JM,?Maggi E,?Romagnani S,?Ville Y,?Piccinni MP. Cytokines and chemokines in follicular fluids and potential of thecorresponding embryo: the role of granulocyte colony-stimulating factor. HumReprod . 2008; 23, 2001.
  9. Macklon NS, Stouffer RL, Giudice LC &Fauser BC. The science behind 25 years of ovarian stimulation for in vitro fertilization. Endocrine Reviews. 2006; 27 170?207Deo et al., 2011
  10. Mansour, R., Ishihara, O., Adamson, G. D., Dyer, S., de Mouzon, J., Nygren, K.- G., Sullivan, E. and Zegers-Hochschild, F. (2014). International committee formonitoring assisted reproductive technologies world report: assisted reproductive technology 2006. Hum. Reprod. 29, 1536-1551.
  11. Mascarenhas, M. N., Flaxman, S. R., Boerma, T.,Vanderpoel, S. and Stevens, G. A.. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med. 2012; 9, e1001356.
  12. Santonocito, M. Santonocito M,?Vento M,?Guglielmino MR,?Battaglia R,?Wahlgren J,?Ragusa M,?Barbagallo D,?Borz? P,?Rizzari S,?Maugeri M,?Scollo P,?Tatone C,?Valadi H,?Purrello M,?Di Pietro C.. Molecular characterization of exosomes and their microRNA cargo in human follicular fluid: bioinformatic analysis reveals that exosomal microRNAs control pathways involved in follicular maturation. FertilSteril . 2014; 102, 1751.
  13. Scott, A. Finn, V. Cardone, I. Hardy, J. Hill, M. Hosseinzadeh. Fertility Centers of New England, Reading, MA. Stimulation Protocol Does not Affect Embryo Quality or Pregnancy Rates: A Prospective Study. L. Fertility &Sterility. 2005; Vol. 84, Suppl 1.
  14. Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, et al. Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for bovine oocyte developmental competence. PLoSOne . 2013;8:e78505.
  15. Sonderegger, S., Pollheimer, J. &Knofler, M. Wntsignalling in implantation, decidualisation and placental differentiation--review. Placenta . 2010;31, 839.
  16. Su L, Zhao S, Zhu M, Yu M. Differential expression of microRNAs in porcine placentas on Days 30 and 90 of gestation. Reproduction, Fertility and Development. 2010; 22:1175-1182.
  17. Tscherner A, Stalker L and LaMarre J. Is there a small RNA fingerprint of embryo quality and health inspent IVF media?Fertility Genetics Magazine. 2015 ? Volume 1 ?P6-8
  18. Wahid F, Shehzad A, Khan T, Young Kim Y. MicroRNAs: Synthesis, mechanism, function, and recent clinical trials. BiochimicaetBiophysicaActa. 2010; 1803:1231?1243.

[Mufeda Ali Jwad, Ban Abbas and Ula Al-Kwaz. (2018); COMPARISON BETWEEN EFFECT OF LONG AGONIST AND ANTAGONIST PROTOCOLS ON MICRO-RNA- 320 LEVEL IN FOLLICULAR FLUID DURING IVF- ICSI. Int. J. of Adv. Res. 6 (12). 01-07] (ISSN 2320-5407). www.journalijar.com


mufedaalammar@yahoo.com
High institute of infertility management and ART

DOI:


Article DOI: 10.21474/IJAR01/8107       DOI URL: http://dx.doi.org/10.21474/IJAR01/8107


Share this article