Protective effect of sulphated polysaccharides and aqueous extract of Ulva lactuca on N-nitrosodiethylamine and Phenobarbital induced nephrotoxicity in Rats

The scope of the present study was to investigate the potential effect of sulphated polysaccharides and aqueous extract of Ulva lactuca against N-nitrosodiethylamine (NDEA) and phenobarbital (PB) induced renal toxicity. However, nephrotoxicity due to NDEA remains one of the most current investigations challenges. In the present study, an attempt has been made to evaluate the potentiality of Ulva lactuca extracts on renal injury markers caused by NDEA. Adult male albino rats were divided into four groups. Three groups (B, C& D) received a single dose of NDEA intraperitoneal (200 mg/kg body weight) for 2, 12 and 24 weeks to provoke nephrotoxicity. Further, the (B, C& D) groups received PB (0.05%) in drinking water after two weeks of NDEA administration, then two (C& D) of which simultaneously received sulphated polysaccharides and aqueous extract of Ulva lactuca, respectively by oral gavage (50 mg/kg body weight) along the entire period of study. Saline (0.9%) treated control group (A) was also built-in. The results revealed that NDEA and PB induced an increase in the blood urea nitrogen (BUN), uric acid and creatinine concentrations; diagnosis of renal damage was normalized by simultaneous administration of sulphated polysaccharide better than the aqueous extract of Ulva lactuca. Moreover, the extent of kidney damage was appraised by histopathological findings. Simultaneous administration of sulphated polysaccharide treatment significantly attenuated the impairment of renal markers by bringing a decrease in their levels, which might be increase the antioxidant status. These results underline the antioxidant property of sulphated polysaccharides and its nephroprotective action against NDEA-induced nephrotoxicity.