03Dec 2018

LISTERIA MONOCYTOGENESIN RAW MILK AND DAIRY PRODUCTS;OCCURRENCE AND TRIALS FOR THEREDUCTION OF THEIR RESISTANCE TO ANTIBIOTICS.

  • Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine,Benha University, 13736, Egypt.
  • Pharmacology Department, Animal Health Research Institute, Dokki, Giza, 12618, Egypt.
  • Biochemistry Department, Faculty of Agriculture, Zagazig University,44511, Egypt.
  • Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt.
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Listeria monocytogenes is a facultative intracellular pathogen responsible for severe infection in animals and humans. This study aimed to determine the occurrence of Listeriamonocytogenes in 200 samples of raw milk and dairy products marketed in Egypt, with detection the antimicrobial resistance profiles of the isolates for different antimicrobial agents. The synergistic effects of three plant extracts in combination with the used antimicrobials to control resistant L. monocytogenes isolates were assessed, with evaluation of relationship between the antibacterial activity and composition of plant extracts. A total of 5 (2.5%) L. monocytogenes isolates were identified (3 isolates from raw milk and two isolates from kariesh cheese and ice cream, one each). The isolates were sensitive to ciprofloxacin and sulphadimidine, while for amoxicillin and oxytetracycline, were resistant. The higher antimicrobial effects of basil and cinnamon extracts were detected, in addition, low concentrations of them were enough to show synergistic effect with the amoxicillin and oxytetracycline to control resistant isolates, these might be attributable to their chemical compositions. In conclusion, there is need for effectively pasteurized raw milk consumption or usage in the production of dairy products. In addition, the chemical composition of these plant extracts might help in its use to control resistant isolates with appropriate antibiotics without the need for increasing the used dosage doses.

  1. Abd El Tawab, A., A. Maarouf and Z. Mahdy (2015): Bacteriological and molecular studies of Listeria species in milk and milk products at El-Kaliobia governorate. BVMJ, 2(29): 170‐
  2. Adwan, G. and M. Mhanna (2008): Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle-East Journal of Scientific Research, 3(3): 134-139.
  3. Ahmed, H. (2013): Public health significance of Listeria as a foodborne pathogen. Zoonoses Department. Faculty of veterinary medicine Cairo University, Egypt.
  4. Al Kassaa, I., K. El Omari, B. Esmail, M. Hamze and M. Saati (2016): Prevalence of Listeria monocytogenes in raw milk in North Lebanon. LSJ, 37-43.
  5. Betoni, J.E.C., R.P. Mantovani, L.N. Barbosa, L.C. Di Stasi and A. Fernandes Junior (2006): Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Mem?rias do InstitutoOswaldo Cruz, 101(4): 387-390.
  6. Brant, R., J. Pinto, S. Bertolucci and C. Albuquerque (2008): Essential oil content of Aloysiatriphylla (L?Her.) Britton in function of seasonal variation. Rev. Bras. Pl. Med, 1083-88.
  7. Chanda, S. and K. Rakholiya (2011): Combination therapy: Synergism between natural plant extracts and antibiotics against infectious diseases. Microbiol Book Series, 520-529.
  8. Chaves, C. and M.L. Arias (2009): Characterization of Listeria monocytogenes isolates obtained from raw cheese samples acquired from different Costa Rican producer zones. Archivoslatinoamericanos de nutricion, 59(1): 66-70.
  9. CLSI (2002): Performance standards?? for antimicrobial susceptibility testing, vol. 22, no. 1. 12th? informational supplement M100-S12. Wayne, PA. Clinical and Laboratory Standards Institute.
  10. CLSI (2012): Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard?Ninth Edition. CLSI document M07-A9. Wayne, PA. Clinical and Laboratory Standards Institute.
  11. CLSI (2016): Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI supplement M100S. Wayne, PA. Clinical and Laboratory Standards Institute.
  12. Dew?, T. (2014): Report on the rapid integrated assessment of nutrition and health risks in small ruminant value chains in Ethiopia. Nairobi, Kenya: ILRI.
  13. Effimia, E. (2015): Prevalence of Listeria monocytogenes and Salmonella spp. Ready-to-Eat Foods in Kefalonia, Greece. J BacteriolParasitol, 6(243): 2.
  14. El-Maati, M.F.A., S.M. Labib, A.M. Al-Gaby and M.F. Ramadan (2016): Antioxidant and antibacterial properties of different extracts of garden cress (Lepidiumsativum L.). Zagazig J. Agric. Biochem. Appl., 43(5): 1685-1697.
  15. Elshikh, M., S. Ahmed, S. Funston, P. Dunlop, M. McGaw, R. Marchant and I.M. Banat (2016): Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. BIOTECHNOL LETT, 38(6): 1015-1019.
  16. G?l?in, İ., Ş. Beydemir, G. Şat and ?.İ. K?frevioğlu (2005): Evaluation of antioxidant activity of cornelian cherry (Cornus mas L.). Actaalimentaria, 34(2): 193-202.
  17. G?l?in, I., E. Bursal, M.H. Şehitoğlu, M. Bilsel and A.C. G?ren (2010): Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food ChemToxicol, 48(8-9): 2227-2238.
  18. Hussien, M.F., M.M. Amin and O.A. Sadek (2013): Comparison between the microbiological quality of kariesh cheese manufactured from raw and pasteurized skimmed milk sold in Assiut city markets. Assiut Vet. Med. J, 59(138): 129-137.
  19. ISO (2004): Microbiology of food and animal feeding stuffs ? Horizontal method for the detection and enumeration of Listeria monocytogenes ? Part 1: Detection method. International Standard Organization ISO 11290-1, AMENDMENT 1: Modification of the isolation media and the haemolysis test, and inclusion of precision data. Geneva, Switzerland.
  20. Jangra, S.S., V.K. Madan and I.D. Singh (2018): Comparative analysis of phytochemical profile and antioxidant activity of coriander (Coriandrumsativum L). Asian J. Chem, 30508-512.
  21. Kamatou, G., A. Viljoen, S. Van Vuuren and R. Van Zyl (2006): In vitro evidence of antimicrobial synergy between Salvia chamelaeagnea and Leonotisleonurus. S Afr J Bot, 72(4): 634-636.
  22. Karadal, F. and Y. Yildirim (2014): Antimicrobial susceptibility and serotype distribution of Listeria monocytogenes isolates obtained from raw milk cheese samples sold in Nigde. studies, 2627.
  23. Kasalica, A., V. Vuković, A. Vranje? and N. Memi?i (2011): Listeria monocytogenes in milk and dairy products. Biotech Anim Husbandry, 27(3): 1067-1082.
  24. Kumar, A., S. Grover and V.K. Batish (2015): Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes. 3 Biotech, 5(3): 261-269.
  25. Marr, K.A., M. Boeckh, R.A. Carter, H.W. Kim and L. Corey (2004): Combination antifungal therapy for invasive aspergillosis. Clin Infect Dis, 39(6): 797-802.
  26. Marzoog, T.R. (2013): Synergistic Effect of Rosmarinusofficinalis Extract with Antibiotics against Different Bacterial Isolates. JET, 31(5 Part (B) Scientific): 678-686.
  27. Meshref, A., M. Zeinhom and N.S. Abdel-Atty (2015): Occurrence and Distribution of Listeria Species in Some Egyptian Foods. AJVS, 46(1).
  28. Morvan, A., C. Moubareck, A. Leclercq, M. Herv?-Bazin, S. Bremont, M. Lecuit, P. Courvalin and A. Le Monnier (2010): Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrob Agents Chemother, 54(6): 2728-2731.
  29. Olajuyigbe, O.O. and A.J. Afolayan (2012): Synergistic interactions of methanolic extract of Acacia mearnsii De Wild. with antibiotics against bacteria of clinical relevance. IJMS, 13(7): 8915-8932.
  30. Ordonez, A., J. Gomez and M. Vattuone (2006): Antioxidant activities of Sechiumedule (Jacq.) Swartz extracts. Food Chem, 97(3): 452-458.
  31. Paparella, A., A. Serio, C. Chaves-L?pez and G. Mazzarrino (2013): Plant-based intervention strategies for Listeria monocytogenes control in foods. Microbial pathogens and strategies for combating them: Science, technology and education, 21230-1246.
  32. Peter, A., E. Umeh, E. Azua and G.A. Obande (2016): Prevalence and Antimicrobial Susceptibility of Listeria monocytogenes Isolated from Beef, Pork and Chicken Sold in Makurdi Metropolis. BMRJ, 14(5): 1-7.
  33. Roginsky, V. and E.A. Lissi (2005): Review of methods to determine chain-breaking antioxidant activity in food. Food Chem, 92(2): 235-254.
  34. Shaaban, H., M. Ahmed, L. Sideek and M. Amer (2013): Study on the antimicrobial activity and Synergistic/antagonistic effect of interactions between antibiotics and some spice essential oils against pathogenic and food-spoiler microorganisms. JASR, 9(8): 5076-5085.
  35. Snigdha, C. and T. Monika (2014): Effect of thermal processing on total phenolic content and antioxidant activity of Coriandrumsativum L. leaves. AJBS, 9(1): 58-62.
  36. Tantawy, H. (2011): Listeria monocytogenes in Egyptian milk and dairy products. Milk Hygiene Faculty of veterinary medicine Alexandria University, Egypt.
  37. Wagner, H. and G. Ulrich-Merzenich (2009): Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine, 16(2-3): 97-110.
  38. Yamaguchi, T., H. Takamura, T. Matoba and J. Terao (1998): HPLC method for evaluation of the free radical-scavenging activity of foods by using 1, 1-diphenyl-2-picrylhydrazyl. BiosciBiotechnolBiochem, 62(6): 1201-1204.
  39. Yildiz, H. (2016): Chemical composition, antimicrobial, and antioxidant activities of essential oil and ethanol extract of Coriandrumsativum L. leaves from Turkey. INT J FOOD PROP, 19(7): 1593-1603.

[Fatma I. El-hofy, Ashraf A. Abd El-tawab, Elham A. Mobarez, Mohamed F. Abo El-Maati, Nancy Y. Tawkol and Ahmed A. Hefny. (2018); LISTERIA MONOCYTOGENESIN RAW MILK AND DAIRY PRODUCTS;OCCURRENCE AND TRIALS FOR THEREDUCTION OF THEIR RESISTANCE TO ANTIBIOTICS. Int. J. of Adv. Res. 6 (Dec). 100-106] (ISSN 2320-5407). www.journalijar.com

Ahmed A. Hefny
Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University

DOI:

Article DOI: 10.21474/IJAR01/8119      
DOI URL: http://dx.doi.org/10.21474/IJAR01/8119

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