01Jan 2019

ANTIMICROBIAL AND GCMS ANALYSIS OF CHLOROFORM EXTRACT OF PSEUDARTHRIA VISCIDA (L.) WIGHT AND ARN. AND ASSOCIATED MAJOR FUNGAL ENDOPHYTE.

  • Department of Forest Pathology, Kerala Forest Research Institute, Peechi-680653, Thrissur, Kerala.
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Pseudarthriaviscidaa member of Fabaceae family possess great medicinal attributes.The present study dealt with antimicrobial ability of chloroform extract P. viscidaand its associated major fungal endophyte and their phytochemical analysis via GCMS. Pseudarthriaviscida yielded two endophytesColletotrichumgloeosporioides (Pv-1) and C. lindemuthianum (Pv-2)and isolation frequency resulted C. gloeosporioides (Pv-1) to be major one. Chloroform extracts at different concentrations (25, 50, 75 and 100μl) ofP. viscidaleaves, mycelial mat (?g/ml)and culture filtrate (v/v) of major endophyte C. gloeosporioides(Pv-1) were tested for their antimicrobial activity against two bacterial species, Escherichia coli and Staphylococcus aureus and two fungal species, Fusariumoxysporum and Cladosporiumcladosporioidesrespectively. The extracts were found to show inhibitory activity against all the studied pathogens. GC-MS analysis of both plant and fungal endophyte resulted in the presence of 16 compounds from P. viscidaplant extracts, 16 from 16 from Pv-1 C.F. and 20 from Pv-1 M.E. In case of P. viscidaand its endophyte Pv-1, 2,4-Ditert-butylphenol, E-14-Hexadecenal, E-15-Heptadecenal, 1-Hexadecene, Cyclotetracosane, Octadecyltrifluoroacetate were similarbut their area percentage varied. Majority of the compounds from extracts were identified to possess therapeutic properties and further chemical evaluation is needed for its successful application.


  1. Arnold, A.E., Maynard. Z., Gilbert, G.S., Coley, P.D. and Kursar, T.A. (2000). Are tropical fungal endophyteshyperdiverse? Ecology Letters, 3, 267-274.
  2. Arx, V. J. A. (1981). The genera of fungi sporulating in pure culture, J. Cramer. In der A.R. GantnerVerlagKommanditgesellschaft. FL ? 9490, Vaduz. 424.
  3. Baccon, C.W. and White, J.F. (2000). Microbial endophytes. Marcel Dekker, New York, pp. 341-388.
  4. Bandara, W. M. M. S., Seneviratne, G., Kulasooriya, S.A. (2006). Interactions among endophytic bacteria and fungi: effectsand potentials. J Biosci 31(5):645-650.
  5. Baskar, N., Jaiganesh,K.P., Nepolean, R. and Selvakumar, N. (2012). Evaluation of antimicrobial activity of methanolic extract of the Pseudarthriaviscida International Journal of Pharmaceutical and Chemical sciences, Vol. 1 (4).
  6. Bezerra, J.D.P., Nascimento, C.C.F., Barbosa, R., Silva, D., Svedese,V., Silva-Nogueria, E.,Gomes, B., Paiva, L. and Souza-Motta, C. (2015). Endophytic fungi from medicinal plant Bauhinia forficate: Diversity and biotechnological potential. Brazilian Journal of microbiology 46,1, 49-57.
  7. Bhagya, N., Sheik, S., Sharma, S.M. and Chandrashekar, K.R. (2011). Isolation of endophyticColletotrichumgloeosporioidesPenz. From Salaciachinensis and its antifungal sensitivity. Journel of Phytology, 3(6):20-22.
  8. Chandra, S. (2012). Endophytic fungi: novel sources of anticancer lead molecules. ApplMicrobiolBiot 95:47-59.
  9. Ellis, M. B. and Ellis, J. P. 2001. Microfungi on land plants an identification handbook. Croom helm Australia Pvt. Ltd., Sydney, Australia. 818.
  10. Gangadevi, V. and Muthumary, J. (2008). Isolation ofColletotrichumgloeosporioides, a novel endophytictaxol- producing fungus from the leaves of a medicinal plant, Jusriciagendarussa. Mycologiabalcanica 5: 1-4.
  11. Gilman, J. C. (1994). A manual of soil fungi. The Iowa state college Press-Ames, Iowa, USA.450.
  12. Hemlal, H. and Ravi, R. (2012). GC-MS, HPTLC and Antimicrobial analysis of Root extracts of PseudarthriaviscidaWight and Arn and Desmodiumgangeticum(Linn) DC.International Research Journal of Biological Sciences 1(5), 57-65.
  13. Hwang, J. S., You, Y. H., Bae, J. J., Khan, S. A., Kim, J. G. and Choo, Y. S. (2011) Effects of endophytic fungal secondary metabolites on the growth and physiological response of CarexkobomugiOhwi J Coastal Res 27:544-548.
  14. Petrini, O. and Fisher, P.J. (1990). Occurrence of fungal endophytes in twigs of Salix fragilis and Quercusrobur.Mycological Research 94: 1077-1080.
  15. Rakotoniriana, E.F., Munaut, F.,Decock, C., Randriamampionona, D., Andriambololoniaina, M., Rakotomalala, T., Rakotonirina, E.J., Rabemanantsoa, C., Cheuk, K., Ratsimamanga, S.U., Mahillon., El-Jaziri, M., Quetin-Leclercq, J. and Corbisier, A.M. (2007) Endophytic fungi from leaves of Centellaasiatica: occurrence and potential interactions within leaves. Springer Science+Business Media B.V.
  16. Ramarao, P. and Manoharachary, C. (1990). Soil fungi from Andhra Pradesh. Department of Botany, Osmania University. 164.
  17. Schulz, B., Boyle, C., Draeger, S., Rommert, A.K. and Krohn, K.(2002). Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res, 106:996?1004.
  18. Selvamohan, T., Ramadas, V. and Kishore, S. (2012). Advance in applied science research, 3(5): 3374-3381.
  19. Shanthakumar, S., Saravanan, C., Anandan, R., Narayanswamy, V. B. and Varunraj, S. (2009). Anti-Pyretic and Anti-Nociceptive activity of Alcoholic extract of Pseudarthriaviscida. The pharmacist 4 (2): 27-30.
  20. Simms, E. L. (1992). Costs of plant resistance to herbivoryin R. S. Fritz, and E. L. Simms (eds.). Ecology and Evolution of Plant Resistance. University of Chicago Press, Chicago. pp. 392?495.
  21. Siqueira, V. M., Conti, R., Araujo, J. M. and Souza-Motta, C. M. (2011). Endophytic fungi from the medicinal plant Lippiasidoides Cham. and their antimicrobial activity. Symbiosis 53: 89-95.
  22. Slutsker, L., Alterkurse, S.F. and Swerdloe, D.L. (1998). Food borne diseases: emerging pathogen and trends. Infect. Dis. clin. North. Am. 12: 199-216.
  23. Souza, A.Q.L., Souza, A.D.L., Astolfi-Filho, S., Pinheiro, M.L.B., Sarquis, M.I.M., Pereira, J.O. (2004). Antimicrobial activity of endophytic fungi isolated from amazonian toxic plants: Palicourealongiflora(aubl.) rich and Strychnoscogens ActaAmaz 34:185-195.
  24. Strobel, G. A. (2002).Microbial gifts from the rain forest. Canadian Journal of Phytopathology, 24: 14-20.
  25. Strobel, G. and Daisy, B. (2003). Bioprospecting for microbial endophytes and the natural products. Microbiology and molecular biology reviews 67 (4), 491-502.
  26. Strobel, G., Daisy, B., Castillo, U. and Harper J. (2004). Natural Products from Endophytic Microorganisms. J Nat Prod, 67(2): 257-68.
  27. Subramanian, C. V. 1983. Hypomycetes. Taxonomy and Biology. Academic Press, London, Vol. I and II. 930.
  28. Teske, M., Trentini, A.M.M. (1995). Comp?ndio de Fitoterapia. Herbarium Lab. Bot?nico, Curitiba.
  29. Zhao, J., Li, C., Wang,W., Zhao, C. and Luo, M. (2013). Hypocrealixii, novel endophytic fungi producing anticancer agent cajanol,isolated from pigeon pea (Cajanuscajan [L] mill sp.). J ApplMicrobiol, 115:102-103.

[Delmy Abraham, Bharath Nair and G. E. Mallikarjunaswamy. (2019); ANTIMICROBIAL AND GCMS ANALYSIS OF CHLOROFORM EXTRACT OF PSEUDARTHRIA VISCIDA (L.) WIGHT AND ARN. AND ASSOCIATED MAJOR FUNGAL ENDOPHYTE. Int. J. of Adv. Res. (1). 105-113] (ISSN 2320-5407). www.journalijar.com


BHARATH NAIR
PHD SCHOLAR

DOI:


Article DOI: 10.21474/IJAR01/8302       DOI URL: http://dx.doi.org/10.21474/IJAR01/8302


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