Antiprotoscolices effects of ethanolic extract of Zingiber officinale against Echinococcus granulosus invitro and invivo

Abstract: Ginger (Zingeber officinale) is one of the world’s best known spices, and it has also been universally used throughout history for its health benefits. The purpose of this study to evaluates the effect of ethanolic extract of Z. officinale (EZO) as antiprotoscolices in vitro and in vivo. Protoscolices of Echinococcus granulosus were collected from sheep livers containing hydatid cyst and were exposed to three different concentrations of EZO extract (150,100,50)mg /ml for 0, 30,60,90, 120 min . The rate of alive of protoscolices was 97% in control group . Protoscolices were exposed to ethanolic extract of Z. officinale at concentration of 50 mg /ml was 60%, 46.2% ,15.37% ,7.7% and 0% after 0, 30, 60 ,90 and 120 min respectively. Viability rate of this extract at concentration 100 mg/ml was 51.33%, 37.53%,20% and 0% after 0,30,60 and 90 min respectively. Zero percent viability rate was observed at concentration of 150 mg/mL after 60 min of exposure. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated with EZO in the next day. The result showed in treated group significant reduction in weight of liver and spleen . Histological sections of the liver from mice treated with EZO at concentration 50 mg /ml showed proliferation of kupffer cells as a defensive cell with few mononuclear cells in sinusoids and absence of hydatid cyst comparing with control group revealed Hydatid cyst wall and necrosis of hepatocytes. These results showed that EZO have protoscolicidal effects in vitro and in vivo.

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[Noor Nihad Baqer, Marwa Hussein Khuder , Nusaibah Amer (2014); Antiprotoscolices effects of ethanolic extract of Zingiber officinale against Echinococcus granulosus invitro and invivo Int. J. of Adv. Res. 2 (10). 0] (ISSN 2320-5407). www.journalijar.com

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