Immunoradiometric Assay for Determination of Thyroid Stimulating Hormone in Human Serum using Solid Phase Anti-TSH Magnetic Particles

Abstract: The preparation and development of primary reagents of thyroid stimulating hormone (TSH) Immunoradiometric (IRMA) technique using solid phase magnetic particles with low cost is considered to be the main objective of the present study. The production of polyclonal antibodies was undertaken by immunizing four male New-Zealand rabbits. The preparation of 125I-TSH antigen and 125I-TSH MoAb tracers was carried out using iodogen. The preparation of 125I-TSH MoAb radiotracers were carried out using Iodogen as oxidizing agent. The preparation of standard solution of TSH was carried out. Activation and coupling of magnetizable particles with purified polyclonal anti-TSH was carried out. Optimization and validation of the assay were carried out. The results obtained provide a highly sensitive specific and accurate IRMA system of TSH. In conclusion this assay could be used in diagnosis of hypothyroidism.

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[H. M. Shafik and N. H. Ebeid (2015); Immunoradiometric Assay for Determination of Thyroid Stimulating Hormone in Human Serum using Solid Phase Anti-TSH Magnetic Particles Int. J. of Adv. Res. 3 (2). 860-867] (ISSN 2320-5407). www.journalijar.com

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