Comparative proteome analysis of Brucella abortus under different growth conditions by two dimensional electrophoresis

Abstract: Brucellosis is one of the most common zoonotic diseases in the world. Bovine brucellosis is majorly caused by Brucella abortus and is widespread in India and appears to be on the increase in recent times. In the present study, proteome of Brucella abortuswas compared under different growth conditions i.e. temperature variations and iron limited conditions by two dimensional electrophoresis. Brucella abortus was cultured at different temperatures (30oC,37oCand 42oC) and iron limited condition by using different concentrations of iron chelator2,2’-Bipyridyl (i.e. 100 µm and 200 µm)in Mueller Hinton agar. The cells were lysed in lysis buffer. Protein fraction was purified by 2DE cleanup kit (GE Healthcare, USA)and subjected to iso-electric focusing. Later, the strips were subjected to 2nd dimension SDS PAGE. The gels were stained with coomassiebrilliant blue. Spots were scanned and analysed using Image master platinum 7. A total of 375, 382 and 363 protein spots were detected for B. abortus at 300C, 370C and 420C respectively. Under heat stress bacterial protein synthesis was altered as it synthesized 25 new proteins (30 - 70 kDa MW range) in response.A total of 380 spots were detected under normal growth conditions, while 356 and 368 spots were detected in bacteria supplemented with 100 µm and 200 µm of 2,2’-Bipyridyl containing media respectively. Around 39 were over expressed and 41 proteins were down regulated at higher iron chelator concentrations.

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[Reddi V.V.S.N. Murthy, Jaspreet Singh Arora and B.V. Sunil Kumar (2015); Comparative proteome analysis of Brucella abortus under different growth conditions by two dimensional electrophoresis Int. J. of Adv. Res. 3 (2). 0] (ISSN 2320-5407). www.journalijar.com

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