Phenotypic and molecular study of antibiotic resistance genes in microbiota isolated from slaughtered ewes uteri

The present study aimed to identify most important microbiota isolated from uteri in ewes and evaluate the phenotypic resistance patterns of these identified microbiota. Molecular study also has been conducted to confirm antibiotic resistance genes. Sixty one of uteri obtained from slaughtered sheep have grossly been examined for any signs of inflammation then under aseptic conditions samples for bacterial culture have been obtained by sterile swabs . the samples were cultured on macConkey agar medium, fourty seven samples only shown a growth. Thirty two of isolates were identified by conventional biochemical tests where the results revealed the following bacteria: Escherichia coli1 14(43.75%) , Klebsiella spp. 9(28.125%), Enterobacter spp. 5(15.625%), Citrobacter spp. 2(6.25%) and Proteus spp. 2(6.25%). The results of antibiotic resistance patterns revealed that 100% of isolates were resist to oxacillin, the resistance to ampicillin and tetracycline were 96.87% and 43.75% respectively moreover the cefamandole and gentamicin were the more active antibiotics against isolates where the percentage of resistance was 0% for both. The present study showed that 21.88% of isolates were carried blatem genes. Our conclusion is that the identified microbiota in this study have phenotypic and genotypic resistance which suggest the acquirement of these bacteria of antibiotic resistance genes which fortify these bacteria against the recommended treatment with antibiotics and increasing the chances to infect the animal genitalia which exacerbate the animal health especially during gestation and parturition.