26Apr 2015

Virulence determinants, drug and metal resistance of clinical and environmental Aeromonas species

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Seventy nine clinical and environmental samples were screened for the presence of Aeromonas species and a total of twenty seven isolates were recovered. Various virulent determinants were screened which showed the presence of Type 3 Secretion System genes (aexT and ascU) and Type 6 Secretion System regulatory protein gene hcp2. Antibiotic susceptibility test revealed resistance to amoxicillin, aztreonam and cephalothin and 21 different patterns of resistance was observed. Screening of TEM1 for ? lactamases showed that 18 isolates carried the respective gene. Over 25 % isolates produced ? haemolysis on 5 % sheep blood agar medium. The Minimum Inhibitory Concentration (MIC) values for silver nitrate were determined to be 250µM and for copper sulphate was 6mM. Resistance genes for silver and copper like silP and copA was screened and determined that 12 isolates and one isolate were positive for each, respectively. Sequencing of 16S rRNA gene (IAH22) showed the isolate was Aeromonas hydrophila. Thus the Aeromonas sp. is considered to be an emerging pathogen and identified as a high-risk carrier.


[U. Indra, M. Sureshkumar, B. Laksman Kumar and G. Vivekanandhan (2015); Virulence determinants, drug and metal resistance of clinical and environmental Aeromonas species Int. J. of Adv. Res. 3 (4). 0] (ISSN 2320-5407). www.journalijar.com


U. Indra, M. Sureshkumar, B. Laksman kumar and G. Vivekanandhan


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